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Small intestine
Effect of proinflammatory interleukins on jejunal nutrient transport
  1. J Hardin,
  2. K Kroeker,
  3. B Chung,
  4. D G Gall
  1. Gastrointestinal Research Group, University of Calgary, Calgary, Alberta, Canada
  1. Dr D G Gall, Faculty of Medicine, Health Science Centre, 3330 Hospital Drive N W, Calgary, Alberta, T2N 4Nl, Canada. Email:dggall{at}ucalgary.ca

Abstract

AIM We examined the effect of proinflammatory and anti-inflammatory interleukins on jejunal nutrient transport and expression of the sodium-glucose linked cotransporter (SGLT-1).

METHODS 3-O-methyl glucose and l-proline transport rates were examined in New Zealand White rabbit stripped, short circuited jejunal tissue. The effects of the proinflammatory cytokines interleukin (IL)-1α, IL-6, and IL-8, IL-1α plus the specific IL-1 antagonist, IL-1ra, and the anti-inflammatory cytokine IL-10 were investigated. In separate experiments, passive tissue permeability was assessed and brush border SGLT-1 expression was measured by western blot in tissues exposed to proinflammatory interleukins.

RESULTS The proinflammatory interleukins IL-6, IL-1α, and IL-8 significantly increased glucose absorption compared with control levels. This increase in glucose absorption was due to an increase in mucosal to serosal flux. IL-1α and IL-8 also significantly increasedl-proline absorption due to an increase in absorptive flux. The anti-inflammatory IL-10 had no effect on glucose transport. The receptor antagonist IL-1ra blocked the ability of IL-1α to stimulate glucose transport. IL-8 had no effect on passive tissue permeability. SGLT-1 content did not differ in brush border membrane vesicles (BBMV) from control or interleukin treated tissue.

CONCLUSIONS These findings suggest that intestinal inflammation and release of inflammatory mediators such as interleukins increase nutrient absorption in the gut. The increase in glucose transport does not appear to be due to changes in BBMV SGLT-1 content.

  • glucose transport
  • small intestine
  • intestinal inflammation
  • inflammation

  • Abbreviations used in this paper

    TTBS
    0.05% Tween 20 Tris buffered saline
    BSA
    bovine serum albumin
    BBMV
    brush border membrane vesicle
    EDTA
    ethylene diaminetetraacetic acid
    SGLT-1
    sodium-glucose linked cotransporter
    IL
    interleukin
    PD
    potential difference
    Isc
    short circuit current
    G
    conductance
    Jms
    mucosal to serosal flux
    Jsm
    serosal to mucosal flux
    Jnet
    net flux
    SDS/PAGE
    sodium dodecyl sulphate-polyacrylamide gel electrophoresis

    • https://doi.org/10.1136/gut.47.2.184

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    • Abbreviations used in this paper

      TTBS
      0.05% Tween 20 Tris buffered saline
      BSA
      bovine serum albumin
      BBMV
      brush border membrane vesicle
      EDTA
      ethylene diaminetetraacetic acid
      SGLT-1
      sodium-glucose linked cotransporter
      IL
      interleukin
      PD
      potential difference
      Isc
      short circuit current
      G
      conductance
      Jms
      mucosal to serosal flux
      Jsm
      serosal to mucosal flux
      Jnet
      net flux
      SDS/PAGE
      sodium dodecyl sulphate-polyacrylamide gel electrophoresis
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