BACKGROUND AND AIM The treatment of ulcerative colitis (UC) with 5-aminosalicylic acid (5-ASA) does not have the same therapeutic effect in all patients. We tested the hypothesis that the effectiveness of the drug is related to its mucosal concentration.
PATIENTS Twenty one UC patients receiving oral 5-ASA (2.4–3.2 g/day) were enrolled in the study. Four were also receiving topical treatment (2 g/day).
METHODS Six endoscopic biopsies were taken from the rectum for measurement of 5-ASA concentrations (ng/mg) by HPLC; soluble interleukin 2 receptor (sIL-2R) concentrations (U/ml) were measured by ELISA and histology. Endoscopic and histological appearance was graded on a four point scale (0–3). The Wilcoxon's rank test and Pearson's correlation coefficient were used for statistical analysis.
RESULTS Mucosal concentrations of 5-ASA were significantly higher (p=0.03) in patients with endoscopic scores of 0–1 compared with those with scores of 2–3 (16.1 (range 10.2–45) v 5.5 (3.5–17.4), respectively) and in patients with lower histological inflammation compared with those with more severe scores (17.4 (10.5–45)v 8.9 (3.5–17.2), respectively) (p<0.01). In contrast, mucosal sIL2-R concentrations were significantly lower in patients with slight endoscopic and histological lesions than in those with more severe disease. A significative inverse correlation (r=−0.85) was found between 5-ASA and sIL-2R mucosal concentrations (p=0.00008).
CONCLUSIONS In patients with UC, in the same area of the intestinal tract, we found that the higher the 5-ASA mucosal concentrations, the lower the IL-2R levels and endoscopic and histological scores. We hypothesise that maintenance of high mucosal 5-ASA concentrations in all colonic segments could contribute to improve clinical outcome in UC patients.
- ulcerative colitis
- interleukin 2
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Ulcerative colitis (UC) is a chronic inflammatory disease of unknown aetiology sustained by an uncontrolled immunoinflammatory response. Clinical findings have shown that 5-aminosalicylic acid (5-ASA) is effective both in the treatment of active disease and in the prevention of recurrence1-4 but its precise mode of action and optimal dosage remain unknown.5-7 Several studies suggest that the drug acts locally and thus several pharmaceutical formulations have been designed to ensure delivery of 5-ASA into the large bowel, the site of inflammatory lesions. In fact, the drug does not gain access to the colonic mucosa through the systemic circulation but is taken up from the intestinal lumen. Differences in tablet delivery systems as well as in intestinal behaviour and colonic segmental transit time may lead to differences in drug availability at the colonic mucosal level8-11 and could explain the interindividual variability in effectiveness often observed with 5-ASA. In fact, in vivo studies have shown that the same oral dose does not always exert the same therapeutic effect and increasing the oral dose does not invariably provide additional therapeutic benefit to all patients.12-16 As a direct dose-effect relationship between 5-ASA and almost all of its therapeutic targets has been clearly demonstrated in vitro,17-20 it is likely that in vivo, the therapeutic effect of 5-ASA depends directly on the actual mucosal concentration obtained in a single patient in a given colonic tract.
To investigate this issue, attempts have been made to correlate endoscopic, histological, and mucosal immunological activities of colitis, detected in a given segment of the colon, with mucosal 5-ASA concentrations measured in the same part of the intestinal tract.
Materials and methods
Twenty one patients (aged 36–68 years; 13 males, eight females) with UC were studied during a programmed follow up involving clinical, endoscopic, and histological assessment. The study included patients with mild to moderate colitic activity as well as those in remission. All patients were receiving oral 5-ASA formulations (2.4–3.2 g/day; Asacol, Bracco, Italy) and four were also receiving topical treatment (2 g/day). None had concomitant immunological, renal, or hepatic disorders or were receiving steroids, immunosuppressives, or antibiotics.
Clinical assessment of colitis was performed according to criteria modified from Lennard-Jones and colleagues.21 Patients with no more than two bowel movements per day and no other signs or symptoms of colitis were defined as in remission (score 0). Mild disease (score 1) included those cases with three to five bowel movements per day or other symptoms of colitis, including rectal bleeding, anorexia, or nausea. Moderate disease (score 3) included more than six, but less than 10, bowel movements per day, with or without rectal bleeding, anorexia, or nausea. Finally, severe disease (score 4) referred to 10 or more bowel movements per day with one or more of the following signs: abdominal tenderness, pulse rate >100 beats/min, fever ( >37.5°C). The endoscopic appearance of colitis was graded (from 0 to 3) according to the presence of oedema, erythema, mucosal exudate, texture, and bleeding.22 The histological degree of inflammation was graded according to the criteria of Morson and Dawson on the following scale: 0 (normal), 1 (slightly active), 2 (moderately active), and 3 (very active).23 Mucosal immunological activity was evaluated by measuring levels of soluble interleukin 2 receptor (sIL-2R).
Patients were prepared for colonoscopy using 3 litres of oral polyethylene glycol solution on the day before the examination, from 4 to 7 pm. This time was chosen to allow patients to take their oral and topical treatment as usual: the last enema of 5-ASA the night before the examination at 11.00 pm and the last tablets at 8.00 am, 2–3 hours before colonoscopy.
All colonoscopies were performed by the same endoscopist who recorded the endoscopic appearance of the rectal mucosa on a separate form. From the same intestinal area, biopsies for measurement of 5-ASA and sIL-2R levels, and conventional histology were taken. Two biopsies for 5-ASA and two for sIL-2R detection were weighed and immediately frozen at −80°C for later assay. Biopsies for histological investigation were processed as usual.
To obtain supernatants, biopsies were treated as previously described.24 Briefly, specimens were gently washed and placed in polypropylene tubes (Becton Dickinson Labware, California, USA) at 0°C at a concentration of 5 mg tissue/ml in 0.5% human albumin/RPMI 1640 (Gibco, Paisley, UK) The specimens were sonicated and supernatants collected by centrifugation (1800g) for 10 minutes. Supernatant samples were aliquoted and stored at −80°C until used.
Mucosal concentrations of 5-ASA were measured using a high performance liquid chromatography method described previously.25 Briefly, analyses were performed on a chromatographic apparatus (Waters, USA) which consisted of a Model 510 solvent delivery system, a Model U6K injector valve, and an electrochemical detector Coulochem (ESA, USA) Model 5100A, equipped with a conditioning cell (Model 5021), an analytical cell (Model 5011), and connected to a Model 746 integrator. After thawing, the biopsy specimen was placed in tubes containing 2 ml of methanol with internal standard. After sonication the supernatants were collected and evaporated to dryness. Samples were reconstituted with 100 μl of mobile phase and aliquots of each sample (5 μl) were chromatographed on an analytical column (Erbasil S C18, 250×4.6 mm id, particle size 10 μM; Farmitalia, Carlo Erba, Italy). The mobile phase was a mixture of 0.01 M Na2HPO4 (pH 3.0) (containing 0.1 mM EDTA, 0.1 M citric acid, and 0.1 mM heptanesulphonic acid) and methanol (85:15, v/v) delivered at a flow rate of 1 ml/min. The standard curve was linear in the selected range with an interassay coefficient of variation of less than 4.6%. Quality control samples were also run on each day of sample analysis. The limit of detection for 5-ASA was 0.1 ng/mg at a signal to noise ratio of 5.
Mucosal concentrations of sIL-2R were measured using an enzyme linked immunosorbent assay test kit (Innotest hIL-2Rs, Innogenetics, Belgium). This assay was based on the dual immunometric sandwich principle and was performed according to the manufacturer's instructions.
Mucosal concentrations of 5-ASA and sIL-2R in patients with low endoscopic and histological activity (score 0–1) were compared with those with more severe disease (scores 2–3) using Wilcoxon's rank sum test for unpaired data. Pearson's correlation coefficient was used to correlate mucosal 5-ASA and sIL-2R concentrations.
Data are reported as median (range). 5-ASA concentrations are expressed as ng/mg of tissue and IL-2R as U/ml of supernatant.
The clinical activity of colitis was mild in seven, moderate in five, and in remission in the remaining nine patients. Nine patients showed a rectal endoscopic picture of moderate colitis (score 2), eight showed minimal signs of inflammation (score 1) while the remaining four patients presented an endoscopic appearance of remission. None showed severe endoscopic signs of disease (score 3). The histological grade of rectal mucosal inflammation was very active (score 3) in two patients, moderately active (score 2) in 10, and slightly active (score 1) in eight. One patient had a normal histological assessment (score 0).
Median mucosal concentrations of 5-ASA were 15.9 ng/mg (range 3.5–45). Patients with slight endoscopic lesions showed significantly (p=0.03) higher concentrations of mucosal 5-ASA than those with a moderate endoscopic score of colitis (16.1 ng/mg (10.2–45)v 5.5 ng/mg (3.5–17.4), respectively) (fig1). Similarly, significant differences (p<0.01) were found for mucosal concentrations of 5-ASA between patients with low histological scores (score 0–1) and those with active histological inflammation (17.4 ng/mg (10.5–45) v 8.9 ng/mg (3.5–17.2), respectively) (fig1).
Soluble IL-2R was detected in all supernatants. Median mucosal concentrations of sIL-2R were 890 U/ml (range 120–1600). Significantly (p<0.01) lower levels of sIL-2R were present in patients with slight endoscopic disease compared with those with moderate endoscopic signs of disease (722 U/ml (120–975) v 1050 U/ml (890–1600), respectively) (fig 2). Similarly, significantly (p=0.03) lower concentrations of sIL-2R were found in specimens with a low histological grade (score 0–1) compared with more inflamed specimens (500 U/ml (120–975) v 945 U/ml (670–1600), respectively) (fig 2).
There was a significant (p<0.001) inverse correlation (r=−0.85) between mucosal concentrations of 5-ASA and sIL-2R (fig 3).
Patients with UC chronically consume 5-ASA to reduce the frequency and severity of clinical recurrence. In spite of treatment, the clinical course of the disease is extremely variable, ranging from periods of prolonged remission to frequent episodes of relapse. Since one possible explanation for the high recurrence rate could be a poor response to treatment, a higher dosage of 5-ASA has been used to provide additional therapeutic benefit to patients. Clinical trials, however, failed to demonstrate a clear cut dose-response relationship for 5-ASA.12-16 26 27
We have demonstrated an inverse relationship between mucosal concentrations of 5-ASA and UC disease activity—that is, in the same part of the intestinal tract, the higher the drug concentration, the lower the endoscopic and histological scores. Moreover, mucosal concentrations of 5-ASA were inversely correlated with mucosal levels of sIL-2R, a marker of mucosal inflammation.
In vitro studies have demonstrated that 5-ASA inhibits the activity of natural killer cells and the synthesis of inflammatory mediators (arachidonates, toxic reactive oxygen metabolites) and cytokines (interleukin 1, tumour necrosis factor α, interferon γ) in a dose dependent manner.28-35 In cell cultures, 5-ASA produced dose dependent inhibition of T cell proliferation by inhibiting IL-2 and IL-2R expression.36 37 In particular, in studies on isolated lamina propria mononuclear cells from patients with inflammatory bowel disease, Pullman and Doe demonstrated inhibition of IL-2 production by increasing 5-ASA concentrations from 0.1 to 2.5 mg/ml.37
We have demonstrated for the first time in vivo that mucosal concentrations of 5-ASA are inversely related to those of sIL-2R, suggesting that the dose related effect of 5-ASA may depend on the concentration in the actual mucosa. Therefore, it is tempting to hypothesise that in patients with UC, if the drug does not reach a given therapeutic mucosal concentration its pharmacological effect is markedly reduced or absent. Indirect confirmation of this hypothesis is given by the effectiveness of 5-ASA enemas in patients with left colitis unresponsive to oral treatment.6 38 39 In fact, it has recently been demonstrated that topical treatment significantly increases mucosal drug concentrations obtained by oral treatment alone.40
The relationship between 5-ASA oral treatment and mucosal concentrations of the drug has not been fully elucidated. Available data indicate that a given oral dose shows high interindividual variability in mucosal concentrations11 and that increasing the oral dose does not lead to a corresponding increase in mucosal concentrations.41 Moreover, 5-ASA concentrates along the entire length of the colon in a heterogeneous fashion. In fact, after oral administration of 5-ASA, the highest concentrations have been detected in the right colon while in the rectum, the site invariably affected by the disease, the amounts were negligible.11 40 These findings indicate that the oral dose is not predictive of colonic distribution and concentrations of 5-ASA. If the relationship between tissue levels of the drug and disease activity emerging from the present investigation is confirmed, methods of obtaining mucosal concentrations of 5-ASA high enough to reach the mucosal therapeutic threshold of the drug should be investigated to offer UC patients more effective treatment.
However, the mucosal therapeutic range of 5-ASA is still unknown and cannot be established from the results of this study. Data emerging from in vitro studies fail to offer a solution to this problem as concentrations of 5-ASA that inhibit cytokines in experimental studies are much higher than the highest mucosal concentrations found in vivo.11 40 42 43 An explanation for this discrepancy could be that in in vitro studies the drug comes into contact with isolated cells, artificially activated by a given stimulus to produce different immunoinflammatory molecules whereas in vivo the drug acts and is distributed in a much more complex system, and inflammatory cells are stimulated in an uncontrolled way by unknown pathogens. Thus it is not surprising that data obtained in vitro do not fully correspond to those in vivo. Further studies to determine the mucosal therapeutic range of 5-ASA are warranted.
Mucosal concentrations of 5-ASA may be influenced by the severity of colonic inflammation. It is well known that colonic inflammation induces histological architectural distortion, epithelial damage, activation and collection of immunoinflammatory cells, and increased mucosal blood flow. These changes may be responsible for impaired mucosal uptake of 5-ASA. However, it is interesting that in two studies, moderate colitis did not impair either 5-ASA uptake or acetylation by colonocytes.44 45 Moreover, it has been demonstrated, by in vivo rectal dialysis, that the drug reduces colonic inflammatory mediators during the active phases of the disease.46 47 Finally, the widely recognised effectiveness of 5-ASA in treating active colitis may indicate that the drug is sufficiently absorbed and concentrated in the inflamed mucosa to exert its therapeutic effect.4 9 42 Thus even if no definitive conclusions can be made, indirect evidence seems to indicate that moderate inflammation does not play a major role in reducing mucosal concentrations of 5-ASA.
In conclusion, this study demonstrates that the endoscopic, histological, and immunological activities of UC are inversely related to mucosal concentrations of 5-ASA, thus suggesting that a poor response to 5-ASA treatment may be related to inadequate mucosal concentrations of the drug.
- Abbreviations used in this paper:
- ulcerative colitis
- 5-aminosalicylic acid
- soluble interleukin 2 receptor
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