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112. EFFECT OF HELICOBACTER GASTRITIS ON GASTRIC ANTIBIOTIC SECRETION IN MAN
  1. P.V. Sherwood1,
  2. J.I.D. Wibawa2,
  3. D.A. Barrett2,
  4. P.N. Shaw2,
  5. D. Jenkins3,
  6. R.C. Spiller1
  1. Depts of 1Gastroenterology, 2Pharmaceutical Sciences and 3Pathology, University Hospital, Nottingham, UK
  1. H.R. Pearce1-1,
  2. N.J. Brown1-1,
  3. K.D. Bardhan1-2,
  4. J.C. Atherton1-3,
  5. N. Kalia1-1
  1. 1-1Surgical & Anaesthetic Sciences, University of Sheffield; 1-2District General Hospital, Rotherham; 1-3Division of Gastroenterology, University of Nottingham, UK

https://doi.org/10.1136/gut.48.suppl_1.A31

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    Introduction: Subgroup analysis ofHelicobacter eradication trials suggests that patients with more severe gastritis have a higher rate of treatment success.

    Aim: To test the hypothesis thatH. pylori gastritis increases gastric secretion rates of metronidazole, amoxicillin and clarithromycin by studying patients before and after treatment.

    Methods: 22 patients and 9 healthy volunteers underwent gastroscopy to diagnose gastritis andH. pylori status. Subjects received a intra-gastric phenol red infusion and an intravenous antibiotic infusion, after a five day pretreatment with omeprazole and an overnight fast. Samples of plasma and gastric juice were obtained for 4 hours and later analysed by HPLC. Pyloric losses were corrected for by phenol red recovery. 9 patients returned at least 6 months later for a gastroscopy to confirm resolution of their gastritis, followed by a further naso-gastric sampling session.

    Results: Gastric clearance of antibiotics (ml/min) (table 1).

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    Abstract 112, Table 1

    Conclusions: Contrary to expectations,Helicobacter gastritis did not significantly increase gastric antibiotic transfer. This may be due to the large inter-subject variability in gastric antibiotic secretion rates, which limits the usefulness of human experiments for examining gastric antibiotic secretion mechanisms. PV Sherwood was supported by an Astra Research Fellowship.

    113. EFFECTS OF GENOTYPICALLY DIFFERENT STRAINS OF HELICOBACTER PYLORI ON HUMAN MICROVASCULAR ENDOTHELIAL CELL PROLIFERATION IN VITRO

    Background/Aims: H. pylori infection is associated with delayed healing of peptic ulcers. Ulcer healing is dependent upon angiogenesis or new blood vessel formation. Proliferation of endothelial cells (ECs) is a crucial process in angiogenesis. This study aimed to determine whether genotypically different strains ofH. pylori inhibited the proliferation of ECsin vitro.

    Methods: Three H. pylori strains were tested on human dermal microvascular ECs (HuDMECs): a cagA+vacA s1/m1 (toxigenic) strain, its VacA- (non-toxigenic) isogenic mutant and a cagA-vacA s2/m2 (non-toxigenic) strain.Campylobacter jejuni andEschericia coli were also tested. An MTT assay quantified overall HuDMEC viability. Dual staining using Hoescht …

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