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  • Expression of human β-defensin 2 (hBD-2) inHelicobacter pylori induced gastritis: antibacterial effect of hBD-2 against Helicobacter pylori

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Helicobacter pylori
Expression of human β-defensin 2 (hBD-2) inHelicobacter pylori induced gastritis: antibacterial effect of hBD-2 against Helicobacter pylori
  1. Y Hamanakaa,c,
  2. M Nakashimaa,b,
  3. A Wadad,
  4. M Itoa,g,
  5. H Kurazonoe,
  6. H Hojof,
  7. Y Nakaharaf,
  8. S Kohnoc,
  9. T Hirayamad,
  10. I Sekinea,b
  1. aDepartment of Molecular Pathology, Nagasaki University School of Medicine, Nagasaki, Japan, bTissue and Histopathology Section, Division of Scientific Data Registry, Atomic Bomb Disease Institute, Nagasaki University School of Medicine, Nagasaki, Japan, cSecond Department of Internal Medicine, Nagasaki University School of Medicine, Nagasaki, Japan, dDepartment of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan, eDepartment of Medical Technology, School of Health Sciences, Okayama University, Okayama, Japan, fDepartment of Industrial Chemistry, Tokai University, Tokai, Japan, gDepartment of Pathology, National Nagasaki Medical Centre, Nagasaki, Japan
  1. Dr M Nakashima, Tissue and Histopathology Section, Division of Scientific Data Registry, Atomic Bomb Disease Institute, Nagasaki University School of Medicine, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan. moemoe{at}net.nagasaki-u.ac.jp

Abstract

BACKGROUND Human β-defensin 2 (hBD-2) plays a role in the innate defence system at mucosal surfaces. Colonisation of Helicobacter pylori in the stomach is an important pathological factor in gastrointestinal illnesses, including gastritis, peptic ulcer, and gastric adenocarcinoma.

AIMS To evaluate the antibacterial role of hBD-2 against H pyloriinfection in the gastric mucosa.

SUBJECTS Biopsied gastric mucosa specimens from H pyloripositive (n=6) and H pylori negative (n=6) individuals were used. H pylori was determined by the presence of urease activity and microscopic examination.

METHODS The specimens were examined for hBD-2 expression by reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemistry, and in situ hybridisation. The antibacterial effect of hBD-2 againstH pylori was evaluated by the number of colony forming units of H pylori after incubation with 0, 10−9, 10−8, 10−7, 10−6, or 10−5 M of hBD-2 peptide.

RESULTS All sixH pylori positive specimens expressed a high level of hBD-2 mRNA while hBD-2 mRNA was not detected in theH pylori negative specimens by RT-PCR. Immunohistochemistry using anti-hBD-2 antiserum revealed that hBD-2 was expressed in the surface epithelium of H pylori infected specimens. In gastric specimens obtained afterH pylori eradication, hBD-2 immunoreactivity had dramatically decreased. In situ hybridisation confirmed that hBD-2 transcripts were localised in the epithelium of H pylori infected gastric specimens. Incubation with hBD-2 reduced the growth rate of cultured H pylori in a dose dependent manner, and incubation with 10−5 M hBD-2 completely inhibited the proliferation of H pylori.

CONCLUSIONS H pylori infection induces hBD-2 expression in the human gastric epithelium. hBD-2 inhibited the growth of H pylori in vitro, suggesting that hBD-2 plays an antibacterial role in H pylori induced gastritis.

  • human β-defensin 2
  • Helicobacter pylori
  • gastritis
  • antimicrobial peptide

https://doi.org/10.1136/gut.49.4.481

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