Article Text

Download PDFPDF

Blisters in the small intestinal mucosa of coeliac patients contain T cells positive for cyclooxygenase 2
Free
  1. H Kainulainen1,
  2. I Rantala3,
  3. P Collin2,
  4. T Ruuska4,
  5. H Päivärinne1,
  6. T Halttunen1,
  7. K Lindfors1,
  8. K Kaukinen2,
  9. M Mäki5
  1. 1Institute of Medical Technology, University of Tampere, Tampere, Finland
  2. 2Medical School, University of Tampere and Department of Internal Medicine, Tampere University Hospital, Tampere, Finland
  3. 3Department of Pathology, Tampere University Hospital, Tampere, Finland
  4. 4Department of Paediatrics, Tampere University Hospital, Tampere, Finland
  5. 5Institute of Medical Technology and Medical School, University of Tampere, Tampere, Finland, and Department of Paediatrics, Tampere University Hospital, Tampere, Finland
  1. Correspondence to:
    H Kainulainen, Institute of Medical Technology, FIN-33014 University of Tampere, Finland;
    heikki.kainulainen{at}uta.fi

Abstract

Background and aims: Coeliac disease is characterised by atrophy of the villi and hyperplasia of the crypts in the mucosa of the small intestine. It is caused by an environmental trigger, cereal gluten, which induces infiltration of the mucosa by inflammatory cells. We hypothesised that these inflammatory cells express cyclooxygenase 2 (COX-2), an enzyme that contributes to the synthesis of pro and anti-inflammatory prostaglandins and is known to be expressed at sites of inflammation in the stomach and colon. We have investigated expression of COX-2 in the coeliac disease affected small intestinal mucosa where it may be an indicator of either disease induction or mucosal restoration processes.

Patients and methods: Small intestinal biopsy samples from 15 coeliac patients and 15 non-coeliac individuals were stained immunohistochemically for COX-2. Samples from 10 of the patients were also stained after these patients had been on a gluten free diet for 6–24 months. Various cell type marker antigens were used for immunohistochemical identification of the type of cell that expressed COX-2. To further verify colocalisation of the cell type marker and COX-2, double immunoperoxidase and immunofluorescence methods were employed. Immunoelectron microscopy was used to investigate the subcellular location of COX-2.

Results: In all samples taken from coeliac patients, clusters of cells with strong immunoreactivity for COX-2 were found in those areas of the lamina propria where the epithelium seemed to blister or was totally detached from the basement membrane. These clusters were reduced in number or totally absent in samples taken after a gluten free diet. No such clusters were seen in any control samples. The density of COX-2 positive cells lining the differentiated epithelium decreased significantly from 13.5 (5.1) cells/105 μm2 (mean (SD)) in the untreated patient samples to 6.5 (2.0) cells/105 μm2 after a gluten free diet (p<0.001), and was 3.3 (1.9) cells/105 μm2 in control samples (p<0.001 compared with untreated or diet treated coeliac samples). Staining for COX-2 was localised to CD3+ T cells and CD68+ macrophages in the mucosal lesions but not all of these cells were positive for COX-2. Immunoelectron microscopy revealed that the ultrastructure of the COX-2 positive cells resembled that of lymphocytes, and the immunoreaction was localised to the rough endoplasmic reticulum and the nuclear envelope.

Conclusions: Our results show that in coeliac disease, blistering of small intestinal epithelial cells is associated with accumulation of COX-2 positive T cells, and the number of these cells decreases after a gluten free diet. These observations suggest that COX-2 mediated prostanoid synthesis contributes to healing of the coeliac mucosa and may be involved in maintenance of intestinal integrity.

  • coeliac disease
  • cyclooxygenase 2
  • mucosal lesion
  • T cells
  • gluten free diet
  • immunohistochemistry
  • APAAP, alkaline phosphatase-antialkaline phosphatase
  • COX, cyclooxygenase
  • DAB, diaminobenzidine
  • IEM, immunoelectron microscopy
  • NFκB, nuclear factor κB
View Full Text

Statistics from Altmetric.com

Footnotes

    Request Permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.

    Linked Articles

    • FILLER
      Ian Forgacs