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  • Hypermethylation of ZNF545 is associated with poor prognosis in patients with early-stage hepatocellular carcinoma after thermal ablation

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Hypermethylation of ZNF545 is associated with poor prognosis in patients with early-stage hepatocellular carcinoma after thermal ablation
  1. Jie Yu1,
  2. Xin Li1,
  3. Qian Tao2,
  4. Xiao-ling Yu1,
  5. Zhi-gang Cheng1,
  6. Zhi-yu Han1,
  7. Mingzhou Guo3,
  8. Ping Liang1
  1. 1Department of Interventional Ultrasound, Chinese PLA General Hospital, Beijing, China
  2. 2Department of Clinical Oncology, The Chinese University of Hong Kong, Hong Kong, Hong Kong
  3. 3Department of Gastroenterology & Hepatology, Chinese PLA General Hospital, Beijing, China
  1. Correspondence to Dr Ping Liang, Department of Interventional Ultrasound, Chinese PLA General Hospital, 28 Fuxing Road, Beijing 100853, China; liangping301{at}hotmail.com; Mingzhou Guo, M.D., Ph.D., Department of Gastroenterology & Hepatology, Chinese PLA General Hospital, #28 Fuxing Road, Beijing 100853, China; mzguo1@hotmail.com

https://doi.org/10.1136/gutjnl-2015-310258

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    We read with interest the article by Wang et al1 on zinc-finger protein 545 (ZNF545) acting as a tumour suppressor gene (TSG) in gastric cancer by inhibiting rRNA transcription and its methylation as a prognostic factor for early stages of gastric cancer. Inactivation of TSGs through promoter hypermethylation also plays an important role in progression of hepatocellular carcinoma (HCC).2–4 We wish to report the results of the expression profile and epigenetic regulation of ZNF545 in HCC, and its methylation role on early-stage HCC progression after thermal ablation.

    Our results showed reduced or loss of ZNF545 expression was found in seven human hepatic cancer cell lines, including SMMC7721, PRF/PCL-5, SK-hep1, HepG2, BEL7402, LAM3 and SNU449, by semiquantitative reverse transcription PCR. Expression of ZNF545 was detected in another cell of HBXF344. The methylation status of the ZNF545 promoter was examined by methylation-specific PCR (MSP). Complete methylation of the …

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    Footnotes

    • JY and XL contributed equally

    • Contributors PL and MG had full access to all of the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis. Study concept and design: PL, JY, MG. Acquisition of data: PL, JY, XL, QT, X-lY, Z-gC, Z-yH and MG. Analysis and interpretation of data: PL, JY, MG. Drafting of the manuscript: JY. Critical revision of the manuscript for important intellectual content: JY, XL.

    • Funding This work was supported by one grant 7144246 from the Beijing Natural Science Foundation, and two grants 81401436 and 81430039 from the National Scientific Foundation Committee of China.

    • Competing interests None declared.

    • Patient consent Obtained.

    • Ethics approval Ethics committee of Chinese PLA general hospital.

    • Provenance and peer review Not commissioned; internally peer reviewed.