Objective To examine the chance of live births and adverse birth outcomes in women with ulcerative colitis (UC) and Crohn's disease (CD) compared with women without inflammatory bowel disease (IBD) who have undergone assisted reproductive technology (ART) treatments.
Methods This was a nationwide cohort study based on Danish health registries, comprising all women with an embryo transfer during 1 January 1994 through 2013. The cohorts comprised 1360 ART treatments in 432 women with UC, 554 ART treatments in 182 women with CD and 148 540 treatments in 52 489 women without IBD. Our primary outcome was live births per ART treatment cycle. We controlled for multiple covariates in the analyses. Our secondary outcomes were adverse birth outcomes.
Results The chance of a live birth for each embryo transfer was significantly reduced in ART treatments in women with UC (OR=0.73, 95% CI 0.58 to 0.92), but not significantly reduced in the full model of ART treatments in women with CD (OR=0.77, 95% CI 0.52 to 1.14). Surgery for CD before ART treatment significantly reduced the chance of live birth for each embryo transfer (OR=0.51, 95% CI 0.29 to 0.91). In children conceived through ART treatment by women with UC, the OR of preterm birth was 5.29 (95% CI 2.41 to 11.63) in analyses including singletons and multiple births; restricted to singletons the OR was 1.80, 95% CI 0.49 to 6.62.
Conclusions Our results suggest that women with UC and CD receiving ART treatments cannot expect the same success for each embryo transfer as other infertile women. Women with CD may seek to initiate ART treatment before needing CD surgery. Increased prenatal observation in UC pregnancies after ART should be considered.
- CROHN'S DISEASE
- IBD CLINICAL
- ULCERATIVE COLITIS
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Significance of this study
What is already known on this subject?
Women with ulcerative colitis (UC) and Crohn's disease (CD) might have decreased fertility due to several factors, including voluntary childlessness, prior surgery, decreased ovarian reserve and increased disease activity.
Assisted reproductive technology (ART) is an appropriate intervention for women with UC and CD who are unable to conceive naturally.
There is little evidence of the efficacy of ART treatment in women with UC and CD. The cumulative live birth rate after six cycles of in vitro fertilisation has only been described in 71 women with UC and 49 women with CD.
No studies have examined the risk of adverse birth outcomes in children after ART treatments in women with UC and CD.
What are the new findings?
In women with UC receiving ART, the chance of a live birth for each embryo transfer is significantly reduced.
UC surgery before ART treatment does not have a negative effect on the chance of live birth for each embryo transfer.
In children conceived through ART treatment by women with UC, the risk of preterm birth is increased.
In women with CD receiving ART, the chance of a live birth for each embryo transfer is decreased (but not significant in the full model).
CD surgery before ART treatment significantly reduces the chance of a live birth for each embryo transfer.
In children conceived through ART treatment by women with CD, there is no increased risk of adverse birth outcomes.
How might it impact on clinical practice in the foreseeable future?
Infertile women with UC and CD may seek to initiate ART treatment more quickly if they cannot conceive naturally as they cannot expect the same success for each embryo transfer as other infertile women.
Women with UC will be reassured that surgery before ART treatment does not affect the chance of a live birth for each embryo transfer.
Increased prenatal observation in UC pregnancies after ART should be considered.
Women with CD may seek to initiate ART treatment before needing CD surgery.
Women with CD will be reassured that children born after ART treatment have normal birth outcomes.
The incidence of ulcerative colitis (UC) and Crohn's disease (CD) continue to increase in many countries and Denmark is one of the countries in the world with the highest reported incidence rate (23 per 100 000 per year for UC and 10 per 100 000 per year for CD).1–5 As the majority of patients with UC and CD are diagnosed in their fertile years,5 clinicians are increasingly faced with issues relating to fertility and pregnancy. Pregnancy attempts in women with UC and CD are most often well planned and patients are often worried about the effects of medical or surgical treatment. Some of the most urgent questions asked by patients of childbearing age concern fertility, safety of a future pregnancy, and risk of adverse birth outcomes.
In the general population in Denmark, up to 10–15% of all women have problems of infertility, leading many to seek assisted reproduction,6 ,7 which includes in vitro fertilisation (IVF), with or without fertilisation with intracytoplasmic sperm injection (ICSI) and transfer of frozen-thawed (FER) embryos. The proportion of women with UC and CD and infertility problems is likely to be at least the same as in the general population or even higher owing to adhesions of the fallopian tubes and ovaries associated with prior abdominal surgery.8 Former studies have found a considerable reduction in postoperative fertility in women with UC requiring ileoanal pouch anastomosis (IPAA),9–12 and after CD surgery.13 ,14 Excluding voluntary childlessness, additional causes of infertility may include increased disease activity and low ovarian reserve.13 ,15 ,16 Serum anti-Mullerian hormone, an indicator of ovarian reserve, is lower in women with CD, especially during periods of active disease, compared with healthy controls.15 ,16
Assisted reproductive technology (ART) is an appropriate fertility intervention for women with UC and CD who are unable to conceive naturally. Therefore, questions about the success of ART treatment in women with UC and CD are highly relevant, especially before surgery for these conditions. There is little evidence of the efficacy of ART treatment in patients with UC and CD. The cumulative live birth rate after six IVF cycles has only recently been described in 71 women with UC and 49 CD women and compared with patients without IBD,17 ,18 but the analyses did not take into account the possible effect of confounding factors which might affect fertility, such as prior surgery, comorbid disease, body mass index (BMI) and tobacco use.
We examined the efficacy of ART in women with UC and CD, compared with women without IBD, on a much larger scale. In this study we used the power of the Danish nationwide registries, which contain all relevant information on ART treatments and births, and compiled data corresponding to a study period of 20 years on unselected patient cohorts. In Denmark, the cumulative proportion of infertility is 16–26% in women who try conceiving for >12 months and more than 15 000 ART treatments are initiated each year.7 For this study, ART refers to traditional IVF, ICSI and FER. The primary outcome studied was live birth and we assessed the chance of a live birth after ART treatments in women with UC and those with CD compared with women without IBD. In subanalyses, we examined the impact of UC and CD surgery before ART treatment. As secondary outcomes, we compared the risk of adverse birth outcomes in children of women with UC and CD and women without IBD after ART treatment.
Materials and methods
Study population and setting
All citizens in Denmark (population approximately 5.6 million inhabitants, >90% Caucasians) have equal and free access to the tax-supported healthcare system, and its uniform organisation allowed us to use a nationwide population-based design. We obtained nationwide Danish data from the following registries: (i) data related to ART treatments and cause of infertility from the Danish ART Registry, (ii) data on the primary outcome of infertility treatment (live birth) from the Danish Medical Birth Registry (MBR), (iii) data from the Danish National Patient Registry (NPR) on comorbid diseases and surgical procedures performed in women with UC and CD and (iv) data from the Central Personal Registration system on death and immigration. Data from all sources were unambiguously linked on an individual level using the unique civil registration number (assigned to all Danish residents at birth from the Central Personal Registration system) and used in all Danish healthcare registries.19
The study population comprised all women (with a valid civil registration number and available for follow-up in Denmark) registered in the ART registry with at least one embryo transfer during the study period of 1 January 1994 to 31 December 2013 (20-year study period). The Danish healthcare system offers infertile couples and single women up to three fully reimbursed IVF or ICSI treatment cycles with fresh embryos and an unlimited number of frozen embryo transfer and insemination cycles (in practice, 3–6 cycles) if the woman is aged <40 years. All treatment cycles for infertility are recorded in the Danish ART registry. The registry was established on 1 January 1994 and includes data on ART treatments in both public and private clinics; registration of all treatments is compulsory.20 ,21 In the ART registry, the embryo transfer to the uterus might be a result of all kinds of preceding treatments (IVF, ICSI, FER).
From the study population, all women with UC and CD were identified in the NPR. The NPR includes records of all patients discharged from Danish hospitals since 1977 and all outpatient visits since 1994.22 ,23 Information in the NPR includes patients’ civil registration numbers, hospital, department, dates of admission and discharge, procedures performed and up to 20 discharge diagnoses based on the International Classification of Diseases (ICD-8 before 1994 and ICD-10 from 1994 onward). All eligible patients who had a discharge history of UC from any hospital in Denmark (ICD-8 codes: 563.19, 569.04; ICD-10 codes: DK51.x (except DK51.9 for unspecified disease)) before the date of embryo transfer were identified. If a woman with UC had a former diagnosis in the NPR with codes of CD she was only included if the latest given diagnosis was UC. Similarly, all eligible patients who had a discharge history of CD (ICD-8 codes: 563.01; ICD-10 codes: DK50.x) before the date of embryo transfer were identified. If a woman with a CD diagnosis had a former diagnosis of UC she was only included if the latest given diagnosis was CD.
For the primary outcome (live birth), the exposed cohorts comprised all ART treatment cycles in women with UC or CD. Because each woman with UC or CD could have several ART treatments, the observation unit was the treatment cycle.24–26
For the secondary outcomes (adverse birth outcomes), the exposed cohorts comprised all children who were conceived through ART treatment by women with UC or CD. Because each woman with UC or CD could have several births and each birth could include several children, the observation unit was the child.
For the primary outcome, the unexposed cohort comprised all ART treatment cycles in women without UC or CD (women without IBD), and the observation unit was the treatment cycle.
For the secondary outcomes, the unexposed cohort comprised all children born after ART treatment by women without IBD, and the observation unit was the child.
Primary and secondary outcomes
The primary outcome was live birth within a period of 124–292 days after the date of each embryo transfer and was identified in the MBR. Thus, a live birth was considered to be the result of the particular ART treatment if the difference was 140–308 days (20–44 weeks)24 from the last menstruation start, corresponding to 124–292 days after embryo transfer. The MBR includes information on all births in Denmark since 1 January 1973 and data are obtained from birth forms, completed by the midwives who attend all births.27 ,28 The forms include data on the mother's civil registration number, date of birth, parity and smoking status during pregnancy, as well as gestational age, birth weight and congenital anomalies (CAs).
Our secondary outcomes included (i) low birth weight (LBW) at term (birth weight <2500 g in children with a gestational age of ≥37 weeks), (ii) preterm birth (birth before 37 completed weeks of pregnancy) and (iii) CAs.
Data on confounders
Covariates were selected a priori. From the NPR we obtained data on comorbid diseases for all women in the study population and a comorbidity index score—the Charlson Index score, was calculated for each treatment cycle of each woman and covers 19 major disease categories weighted according to their prognostic impact.29 We computed the index based on diagnoses recorded during all previous admissions to hospital since 1977, and two index levels were defined: no comorbidity (Charlson Index 0), some comorbidity (Charlson Index ≥1). From the MBR we obtained data on parity (0, +1) and smoking during pregnancy (yes/no). The ART registry did not include information on all confounders during the entire study period but for that period we had data on women's age at time of embryo transfer (continuous variable), calendar year of infertility treatment (1994–1998, 1999–2003, 2004–2008, 2009–2013), type of preceding treatment (IVF, ICSI, FER) and cause of infertility (female factor, male factor, or mixture of factors/idiopathic). From 2006, the ART registry also included information on BMI (calculated as body weight divided by height squared and categorised according to the WHO classifications as underweight (<18.5 kg/m2), normal weight (18.5–24.9 kg/m2), overweight (25.0–29.9 kg/m2) and obese (≥30 kg/m2)), partner's age, alcohol intake (yes/no) and smoking at the time of embryo transfer (yes/no).
For women with UC we calculated the duration of UC at the time of each ART treatment (calculated from the date that the first diagnosis of UC appeared in the NPR, unless they had a later diagnosis of CD, until the date of embryo transfer in the ART registry). Likewise, the disease duration for CD women was calculated from the date that the first diagnosis of CD appeared in the NPR, unless they had a later diagnosis of UC, until the date of embryo transfer.
For women with UC and CD we identified those who had undergone surgery before the date of embryo transfer. Identified were women with UC who had relevant operations according to the following codes in the new and former version of the “Nordic Classification of Surgical Procedures”: codes of KJFH; that is, all types of colectomies; and colectomy codes of 45020, 45060, 45080, 45840, or 45880. For CD women the following codes were used: KJFB00, KJFB01, KJFB20/21/30/31, KJFB33, KJFB34, KJFB40/41/43/44/46/47/50/51/60/61/63/64, KJFB96, KJFB97, KJFH00/01/10/11/20/96, KJGB00/01/10/11/30/31, KJFA60, 43440, 43460, 43520, 43540, 43680, 43700, 43740, 43760, 43780, 43800, 43820, 43840, 43860, 43880, 44060, 44120, 44150, 44160, 44790, 44900, 44920, 44940, 44960, 44980, 45020, 45060, 45080, 45100, 45120, 45200, 45240, 45320, 45480, 45690, 45840, 45860, 45880, 46290. The number of UC- or CD-related surgeries was recorded before each treatment cycle.
Live birth (primary outcome)
Contingency tables were constructed for the main study variables according to the exposed and unexposed cohorts. We used multilevel logistic regression analyses to compute crude and adjusted relative risk estimates (prevalence OR with 95% CIs) for live births following ART treatments in women with UC and CD relative to women without IBD and the model accounted for multiple embryo transfers in the same woman. Adjustment was made for Charlson Index, women's age at the time of embryo transfer, calendar year of infertility treatment, type of infertility treatment and cause of infertility. In an extended model, including data from 2006, we further adjusted for BMI, partner's age, smoking at the time of embryo transfer and alcohol.
In a sensitivity analysis we included only first time ART treatment for each woman with UC or CD and only if they had no missing information on BMI, smoking at the time of embryo transfer and alcohol.
Subanalysis on the impact of UC or CD surgery on the chance of live birth
A possible impact of UC or CD surgery before infertility treatment was examined in women with UC or CD, respectively. Thus, for women with UC who had received ART treatments, two subcohorts were established: (a) an exposed cohort who had had at least one UC-related surgery before the date of embryo transfer and (b) an unexposed cohort who had not had surgery before the date of embryo transfer. Live birth in the exposed cohort (a) was compared with that in the unexposed cohort (b). Similarly, for women with CD who had received ART treatments, exposed and unexposed cohorts were established. In multilevel logistic regression models we adjusted for the duration of UC or CD, Charlson Index, women's age, calendar year of infertility treatment, type of infertility treatment and cause of infertility. Owing to lack of power it was not possible to adjust for smoking at the time of embryo transfer, BMI and alcohol.
All surgery codes given to women with UC at the three latest operation dates before ART treatment were recorded according to the following categories: (i) ‘partial colon resection (no ostomy)’: 45020, (ii) ‘total colon resection (no ostomy)’: 45060, KJFH00, KJFH96, (iii) ‘total colon resection+ileostomy (rectum left in place)’: KJFH10, KJFH11, (iv) ‘rectal/anus resection’: 45080, 45840, 45880, KJFH20, (v) ‘ileal pouch anal anastomosis’: KJFH33. All CD surgery codes from the three latest operation dates before the first embryo transfer were identified and the surgery codes were categorised according to (i) ‘ileocecal resection’: 43700, KJFB20, KJFB21, (ii) ‘ileocolonic resection (more than ileocecal)’: 45060, 43860, 43820, 44900, 43800, 43880, 43840, KJFB33, KJFB30, KJFH00, (iii) ‘small bowel resection or stricturoplasty’: 43680, 44060, 44790, 43780, 43760, 43440, 44120, KJFB00, KJFA60, (iv) ‘stomas but rectum left in place’: 43740, 45240, KJFH10, KJFB60, (v) ‘colon resection’: 44980, 44940, 45120, 44960, 44920, 45320, 45020, KJFB43, KJFB40, KJFB46, (vi) ‘rectal/anus resection’: 45880, 45840, 45080, 45860, KJGB30, KJFH20.
Adverse birth outcomes (secondary outcomes)
We used multilevel logistic regression analyses, accounting for multiple children by the same woman, to compute crude and adjusted relative risk estimates for adverse birth outcomes (LBW at term, preterm birth and CAs) after ART treatment in children of women with UC compared with children of women without IBD; a similar comparison was made for the children of women with CD. The risk estimates of LBW at term, preterm birth and CAs were adjusted for mother's age, parity, calendar year of infertility treatment, type of infertility treatment, cause of infertility and smoking during pregnancy. Estimates of LBW at term and CAs were further adjusted for singleton/multiple births.
In a subanalysis we used multilevel logistic regression to compare the risk of adverse birth outcomes after ART treatment among singletons of women with UC with singletons of women without IBD; a similar comparison was made for singletons of women with CD.
All analyses were conducted using Stata V.13 software (StataCorp LP, College Station, Texas, USA).
Table 1 shows the characteristics for all ART treatments in exposed and unexposed cohorts. A total of 1360 ART treatments in 432 women with UC and 554 ART treatments in 182 women with CD were included during the study period of 1 January 1994 to 31 December 2013. The unexposed cohort included 148 540 ART treatments in 52 489 women without IBD. The median age of women and the distribution according to BMI and smoking were similar in the exposed and unexposed cohorts, whereas fewer women with CD consumed alcohol than women with UC and unexposed women. In the UC cohort, the cause of infertility was referred to as a female factor in 34.5% of cases and a mixture of factors in 44.5% of cases; the corresponding figures for the CD cohort were 46.6% and 36.9% and for the unexposed cohort 30.1% and 38.0%, respectively. The vast majority of women in all cohorts had no comorbid diseases. Among embryo transfers in patients with UC, the median duration of UC at the time of embryo transfer was 8 years (25–75 centiles: 4–12) and surgery had been performed before the transfer in 34.5% of cases. For patients with CD, the median duration of CD at the time of embryo transfer was 9 years (25–75 centiles: 4–13) and surgery had been performed before the transfer in 64.1% of cases.
Live born children (primary outcome)
The ORs for ART treatment in women with UC or CD leading to a live birth, compared with women without IBD, are given in table 2. The chance of a live birth was significantly reduced for each ART treatment in women with UC, with a crude OR=0.79 (95% CI 0.67 to 0.92). Two different models of adjustment were used but the ORs were virtually unchanged from the crude OR (in the fully adjusted model, OR=0.73, 95% CI 0.58 to 0.92). The chance of a live birth was significantly reduced for each ART treatment in women with CD according to the crude OR (0.62, 95% CI 0.48 to 0.80). In the fully adjusted model the chance of a live birth was still reduced but not significantly (OR=0.77, 95% CI 0.52 to 1.14). In the model containing no lifestyle factors, the OR was significantly reduced (table 2).
In the sensitivity analyses, we included only first-time ART treatment for each woman with UC and CD and only if she had non-missing information on lifestyle factors (BMI, smoking at the time of embryo transfer, alcohol). For women with UC, the result of this analysis was similar to the overall result of live birth (adjusted OR=0.79, 95% CI 0.53 to 1.18), and for CD also, the result was similar to the overall result, with a decreased, but not significant, chance of live birth.
Subanalysis of the impact of UC or CD surgery on the chance of live birth
Overall results for a possible impact of UC or CD surgery before infertility treatment on the chance of a live birth are given in table 3. For UC, the results suggested no negative impact of former surgery on the chance of live birth. The result was very robust across the crude and adjusted OR (crude OR=0.92, 95% CI 0.64 to 1.31 and adjusted OR=0.91, 95% CI 0.61 to 1.36). We examined all types of UC-relevant surgical procedures on the three latest dates of operations for all UC patients before their first ART cycle. We found that 195 procedures were performed and with a distribution as follows (the most common procedure first): (a) total colon resection (no ostomy), N=60 (30.8%); (b) total colon resection+ileostomy (rectum left in place), N=53 (27.2%); (c) rectal/anus resection, N=45 (23.1%); (d) ileal pouch anal anastomosis, N=36 (18.5%) and (e) partial colon resection (no ostomy), N=1 (0.51%).
For CD, the results suggested that former surgery had a negative impact on the chance of live birth (crude OR=0.56; 95% CI 0.33 to 0.93 and adjusted OR=0.51; 95% CI 0.29 to 0.91). For women with CD, the three latest operation dates before the first embryo transfer were identified; 206 procedures were performed with a distribution as follows: (a) ‘ileocecal resection’, N=50 (24.3%); (b) ‘ileocolonic resection (more than ileocecal)’, N=44 (21.4%); (c) ‘small bowel resection or stricturoplasty’, N=42 (20.4%); (d) ‘stomas but rectum left in place’, N=26 (12.6%); (e) ‘colon resection’, N=23 (11.2%) ‘and (f) rectal/anus resection’, N=21 (10.2%).
Adverse birth outcomes (secondary outcomes)
Details of adverse birth outcomes are given in table 4 for singletons and multiple births. Children of women with UC had a significantly increased risk of preterm birth in comparison with children of women without IBD (OR=5.29, 95% CI 2.41 to 11.63). The relative risks of LBW at term and CAs were OR=1.48 (95% CI 0.69 to 3.13) and OR=1.52 (95% CI 0.87 to 2.64), respectively. Children of women with CD had no significantly increased risk of preterm birth, LBW at term, or CAs in comparison with children of women without IBD (preterm birth: adjusted OR=0.44; 95% CI 0.09 to 2.21; LBW at term: adjusted OR=1.41; 95% CI 0.40 to 4.89; CAs: adjusted OR=0.59; 95% CI 0.19 to 1.79).
In a subanalysis we estimated the risk of adverse birth outcomes among singletons only and the results are given in table 5. Among singletons delivered by women with UC, the relative risks for LBW at term and CAs were close to unity and the relative risk of preterm birth was OR=1.80 (95% CI 0.49 to 6.62). For children delivered by women with CD, we found no significantly increased risk of preterm birth, LBW at term or CAs.
In this nationwide study with data from a span of 20 years, we found that ART treatments in women with UC and CD led to a reduced chance of a live birth per cycle, compared with ART treatments in women without IBD. Comparison of embryo transfers in women with CD who had had surgery before infertility treatment with those without prior surgery, showed a decreased chance of live births in women who had had surgery. After ART, the risk of preterm birth for children of women with UC was increased fivefold compared with children of women without IBD, but the risk disappeared when singletons only were evaluated.
This is the first study, based on nationwide data, analysing the chance of live birth after ART treatment in women with UC and CD. Earlier, the outcome of ART treatment was studied in 71 women with UC and 49 with CD from two tertiary care centres,17 ,18 who were compared with 470 controls from the general infertile population. It was found that the proportion of live births after the first ART cycle was similar among controls (30.2%) and patients with UC (33.8%) and CD (30.6%). Our overall results differ from these findings. We found that among 1360 embryo transfers in women with UC and 554 embryo transfers in women with CD, there was a reduced chance of a live birth per cycle, compared with 148 540 embryo transfers in women without IBD. It is worth noticing that this result is robust as the crude OR and the ORs from two adjusted models in both UC and CD were close. It might be argued that our finding of a decreased chance of a live birth per cycle in women with UC and CD might not be related to the IBD itself but rather affected by IBD-related factors such as disease activity and/or medications used to treat IBD. The results in our study do not indicate which of these is the cause. It is unlikely that disease activity, at least at the stages of fertilisation and implantation, is the factor responsible for our results as women with IBD in Denmark are not referred for ART treatment unless they have quiescent disease. However, since this is a large register-based study and it was not possible to review individual charts, we cannot rule out the possibility of a negative effect of active IBD, especially during a pregnancy. In addition, we were unable to examine medication use in our patients with IBD. Even if we had obtained information on IBD medication, we could not have adjusted for this in our main analyses as similar medication was not used in the unexposed cohort. Furthermore, as far as we can determine, no studies have indicated that underlying maternal medical treatment affects the result of ART treatments. It is reassuring that in several studies adalimumab, given before embryo transfer, improves the success of ART in women with immune-mediated infertility.30–32
Some results are emerging on the rates of adverse conception and pregnancy outcomes in general, without the use of ART, in women with IBD. Mahadevan's study of the Kaiser database in northern California33 and another recent meta-analysis34 indicated that women with IBD have adverse conception and pregnancy outcomes as well as an increased risk of pregnancy complications. The outcome of live birth can be affected at the stages of fertilisation, implantation and maintenance of the pregnancy throughout each trimester, but there is little knowledge of the factors that reduce the chance of a live birth or the most vulnerable stage (or stages) after ART treatment in women with IBD. Possibly, women with UC and CD have subclinical inflammation or increased immune factors not yet identified,35 such as antiphospholipid, antinuclear, antithyroid and antisperm antibodies or other undiscovered autoantibodies that decrease fertility; these underlying maternal factors may affect the chance of a live birth after ART.
In addition, initial research is now being done on microbiome changes during pregnancy in women without IBD. One study including 91 pregnant women showed significant changes in the gut microbiota during pregnancy.36 Specifically, Koren et al37 observed an overall increase in Proteobacteria and Actinobacteria from the first to third trimester, a decrease in Faecalibacteria and a decrease in the diversity of the microbiota. During pregnancy, a woman's immune system must adapt to avoid rejection of the fetus. At this time, the relationship between the observed changes in the microbiome and the immune system and the role of the bacteria are not known. It has been hypothesised that the changes in microbiome may promote weight gain or may stimulate the innate immune response; the latter may affect the disease course during pregnancy. In addition, the impairment of innate immunity that has previously been reported in IBD may improve during pregnancy with changes in the microbiome and hormones that affect this immune response. The mechanism by which disease inflammation may be increased or decreased during pregnancy is not entirely understood.38 ,39 In pregnancy, the T helper 2 cell (Th2) responses are increased. CD is typically characterised by an exaggerated CD4T helper 1 (Th1) cell response. The increased Th2 response in pregnancy may affect the Th1 response that is upregulated in CD, resulting in a decreased risk of flare. Since UC is a Th2-driven disease and Th2 responses are increased during pregnancy, patients with UC may have an increased risk of a flare.40
For the impact of surgery, it was suggested in the study by Pabby et al,17 based on the same 71 patients with UC as in the study by Oza et al,18 that the cumulative live birth rate after six ART cycles was comparable in women with UC with (22 patients), and without, IPAA (49 patients), but the study was descriptive and did not take confounders into consideration. In our study, 469 embryo transfers in women with UC and prior surgery were compared with 891 embryo transfers in women with UC without prior surgery and both the crude and the adjusted OR were close to unity, indicating that former surgery for UC did not have a negative impact on the chance of a live birth per cycle. The effect of surgery for CD on the chance of live birth after ART treatment has not been studied previously. In our study, 355 embryo transfers in women with CD and prior surgery were compared with 199 transfers in women with CD without prior surgery and both the crude and adjusted OR showed a significant decrease in live births after CD surgery. There are several possible explanations for these results. First, women with UC might have an increased risk of a flare during pregnancy owing to the increased Th2 response. An IPAA or ileostomy is a type of ‘cure’ which removes all present inflammation that might affect pregnancy. Therefore surgery in women with UC might not have a negative effect on live birth, especially since ART bypasses pelvic adhesions. Conversely, surgery for CD often does not remove all inflammation and CD commonly recurs after surgery. Possibly, also, patients with CD who need surgery have increased disease severity, which would account for the worse outcomes after ART treatment. These results are important when counselling women with UC and CD about surgery, if they are also considering a pregnancy.
Our study is the first to examine the risk of adverse birth outcomes in children after ART treatment in women with UC and CD and therefore our results cannot be compared with others. Our results suggest a significant fivefold increased risk of preterm birth in children after ART treatment in women with UC when the OR was adjusted for important confounders such as women's age, parity, type of ART treatment, calendar year of treatment, cause of infertility and smoking during pregnancy. Analysis of the risk of preterm birth in children among only singletons showed no significant increase. We did not find a significantly increased risk of LBW at term or CAs. Women with CD did not have an increased risk of adverse birth outcomes after ART treatment.
The precision and validity of our results depend on the size of the study, accurate classification of exposure, quality of outcome data and the ability to take into account the influence of confounders. According to these aspects our study has several strengths.
First, the study is based on a nationwide study population of all Danish women with UC and CD who had received ART treatment (and all other ART-treated women as controls) as we had access to the ART registry, which is based on mandatory reporting of all individual treatment cycles.
Second, the nature of the health registries allowed a 20-year study period and a large cohort of ART treatments, providing us with high statistical power.
Third, the data from the healthcare registries have a high degree of completeness and validity—that is, according to exposure assessment (diagnoses of UC and CD). The completeness of diagnoses of UC and CD has been examined in a Danish study using the pathology system as a reference standard. This showed that of all patients with a confirmed diagnosis of UC or CD, 94% were included in the NPR, which we had access to, and included obligatory registration of UC and CD diagnoses from both hospitals and outpatient visits.41 Furthermore, the overall validity of diagnosis of UC in the NPR was 94% and of CD 97% in patients diagnosed at specialised departments.41 For our outcome measurements (live birth and adverse birth outcomes), the data from the MBR have a high degree of completeness and validity as all newborn children are registered in the MBR.28
Fourth, we had information on several confounders, including the Charlson comorbidity Index, women's age, calendar year of treatment, type of infertility treatment, cause of infertility, BMI, partner's age, smoking at the time of embryo transfer, smoking during pregnancy and alcohol. Data on the type of operations performed in the patients with UC and CD are of high quality, as data from the NPR on surgical procedures have positive predictive values of 94–100%.
Finally, our outcome data were obtained independently of the hypotheses investigated and exposure measurement, preventing differential misclassification of the outcome measurements.
The study also has some limitations. We lacked detailed information on the severity of UC and CD and the specific location of UC and CD lesions, although such information would only be relevant for the analysis comparing subcohorts of women with UC or CD. As discussed above, we had no information on disease activity and medical treatment for underlying maternal diseases in our cohorts. A further limitation is that we were unable to assess adverse birth outcomes among stillborn or very early abortions (including those due to antenatal diagnoses of CAs). Thus we might have underestimated the ‘true’ relative risks, if CAs after ART treatments, ending in stillbirth or very early abortions, are more prevalent in women with UC and CD than in women without IBD.
We conclude that women with UC and CD have a reduced chance of a live birth per ART treatment cycle compared with women without IBD. In addition, women with CD, but not women with UC, with prior surgery have a decreased chance of a live birth per cycle. These findings might be related to the disease itself or to factors closely related to UC and CD, and additional studies are needed in this area. Our results further suggest that increased prenatal observation in UC pregnancies after infertility treatment should be recommended. It is hoped that these results will inform treatment decisions about the use of ART in UC and CD and, particularly, recommendations for CD surgery and timing of attempts at conception. Future research will hopefully elucidate the mechanism of UC, CD and CD surgery on conception and maintenance of pregnancy. Also, to help with counselling, it would be interesting to examine the “take home chance” of having a baby per patient where the analytical unit is the woman and not the cycle.
Contributors BMN: funding, conception, design, data collection, assistance with data analysis, interpretation of results, manuscript writing and editing, approved the final version. PVL: design, data collection, data analysis, interpretation of results, manuscript editing, approved the final version. JF: assistance with data analysis, interpretation of results, manuscript editing, approved the final version. MDL: interpretation of results, manuscript editing, approved the final version. PSdS: funding, conception, manuscript editing, approved the final version. SF: funding, conception, interpretation of results, manuscript writing and editing, approved the final version.
Funding The study was supported by a Crohn's & Colitis Foundation of America (CCFA), Senior CCFA Research Award Number 112544.
Competing interests None declared.
Ethics approval The study was approved by the Danish Data Protection Agency (j.nr. 2014-41-3466). According to Danish law, no ethical approval of register-based studies is necessary.
Provenance and peer review Not commissioned; externally peer reviewed.