Article Text

Download PDFPDF
Identification of an epithelial member of the protease family, supporting the potential diagnostic and therapeutic role of serine proteases in IBS
  1. Benedicte Y De Winter
  1. Correspondence to Professor Dr Benedicte Y De Winter, Laboratory of Experimental Medicine and Pediatrics, Division of Gastroenterology, University of Antwerp, Universiteitsplein 1, Antwerp 2610, Belgium; benedicte.dewinter{at}

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.

The quest for appropriate diagnostic tools and therapies for IBS runs in parallel with the different pathogenic mechanisms investigated. One of these mechanisms is visceral hypersensitivity, resulting from a disturbed neuronal signalling at the peripheral and/or central levels.1 ,2 Rolland-Fourcade et al 3 show, in this issue of Gut, that intestinal epithelial cells (Caco-2 cells) release a trypsin-like activity at the basolateral side of the cells after stimulation with a classical inflammatory stimulus (lipopolysaccharide) or with a stress-related stimulus (epinephrine), and they identified it as trypsin-3. They confirmed the clinical relevance of trypsin-3 by proving its presence in colonic tissue from patients with IBS (all subgroups). Besides, trypsin-3 increased epithelial permeability in the Caco-2 monolayers and increased the excitability of mouse dorsal root ganglia neurons in vitro. Moreover, it induced Ca+2 transients in human submucosal neurons in vitro and caused visceral hyperalgesia in response to colorectal distension in two rat models of visceral hypersensitivity.3 The novelty of this manuscript lies within the identification of a specific protease, namely trypsin-3 and its source, the intestinal epithelium, providing additional evidence for serine proteases and protease-activated receptors (PAR).

The importance of visceral hypersensitivity as a key contributor to the generation of symptoms in patients with …

View Full Text


  • Competing interests None declared.

  • Provenance and peer review Commissioned; internally peer reviewed.

Linked Articles

  • Neurogastroenterology
    Claire Rolland-Fourcade Alexandre Denadai-Souza Carla Cirillo Cintya Lopez Josue Obed Jaramillo Cleo Desormeaux Nicolas Cenac Jean-Paul Motta Muriel Larauche Yvette Taché Pieter Vanden Berghe Michel Neunlist Emmanuel Coron Sylvain Kirzin Guillaume Portier Delphine Bonnet Laurent Alric Stephen Vanner Celine Deraison Nathalie Vergnolle