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Original article
Microbiota fermentation-NLRP3 axis shapes the impact of dietary fibres on intestinal inflammation
  1. Vishal Singh1,
  2. Beng San Yeoh2,
  3. Rachel E Walker3,
  4. Xia Xiao3,
  5. Piu Saha1,
  6. Rachel M Golonka1,
  7. Jingwei Cai4,
  8. Alexis Charles Andre Bretin5,
  9. Xi Cheng1,
  10. Qing Liu4,
  11. Michael D Flythe6,
  12. Benoit Chassaing5,7,
  13. Gregory C Shearer3,
  14. Andrew D Patterson4,
  15. Andrew T Gewirtz5,
  16. Matam Vijay-Kumar1,8
  1. 1 Department of Physiology and Pharmacology, College of Medicine and Life Sciences, University of Toledo, Toledo, Ohio, USA
  2. 2 Nutritional Sciences, Graduate Program in Immunology and Infectious Diseases, Pennsylvania State University, University Park, Pennsylvania, USA
  3. 3 Department of Nutritional Sciences, Pennsylvania State University, University Park, Pennsylvania, USA
  4. 4 Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, Pennsylvania, USA
  5. 5 Center for Inflammation Immunity and Infection, Institute for Biomedical Sciences, Georgia State University, Atlanta, Georgia, USA
  6. 6 USDA-Agriculture Research Service, University of Kentucky Campus, Lexington, Kentucky, USA
  7. 7 Neuroscience Institute, Institutefor Biomedical Sciences, Georgia State University, Atlanta, Georgia, USA
  8. 8 Department of Medical Microbiology and Immunology, University of Toledo, Toledo, Ohio, USA
  1. Correspondence to Dr Matam Vijay-Kumar, Department of Physiology and Pharmacology, College of Medicine and Life Sciences, University of Toledo, Toledo, OH 43614, USA; MatamVijay.Kumar{at}utoledo.edu

Footnotes

  • Contributors VS conceived the project, designed and performed the mouse experiments. Original observation was made by VS. BSY helped with histochemical staining and writing the manuscript. REW, GCS, JC, QL and ADP analysed SCFA in caecal contents and provided inputs for data interpretation. ACAB, XX, RMG and PS performed colonic gene expression and immune cell profiling. VS, BC and XC generated and analysed the microbiota data. MDF provided hops β-acid with intellectual inputs on bacterial fermentation. ATG assisted with data interpretation and study design. MV-K participated in the study design and directed the study. VS, ATG and MV-K wrote the manuscript.

  • Funding This work was supported by National Institutes of Health grants DK097865 (to MV-K), DK083890 (to ATG) and DK099071 (to ATG). VS is supported by research fellowship award (ID# 418507) and career development award (ID# 597229) from Crohn’s & Colitis Foundation (CCF). PS is supported by research fellowship award from CCF. BC is supported by a career development award fellowship from CCF and by an Innovator Award from the Kenneth Rainin Foundation.

  • Competing interests None declared.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Patient consent for publication Not required.

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Footnotes

  • Contributors VS conceived the project, designed and performed the mouse experiments. Original observation was made by VS. BSY helped with histochemical staining and writing the manuscript. REW, GCS, JC, QL and ADP analysed SCFA in caecal contents and provided inputs for data interpretation. ACAB, XX, RMG and PS performed colonic gene expression and immune cell profiling. VS, BC and XC generated and analysed the microbiota data. MDF provided hops β-acid with intellectual inputs on bacterial fermentation. ATG assisted with data interpretation and study design. MV-K participated in the study design and directed the study. VS, ATG and MV-K wrote the manuscript.

  • Funding This work was supported by National Institutes of Health grants DK097865 (to MV-K), DK083890 (to ATG) and DK099071 (to ATG). VS is supported by research fellowship award (ID# 418507) and career development award (ID# 597229) from Crohn’s & Colitis Foundation (CCF). PS is supported by research fellowship award from CCF. BC is supported by a career development award fellowship from CCF and by an Innovator Award from the Kenneth Rainin Foundation.

  • Competing interests None declared.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Patient consent for publication Not required.

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