Article Text

Download PDFPDF
Original article
STING signalling protects against chronic pancreatitis by modulating Th17 response
  1. Qinglan Zhao1,
  2. Murli Manohar1,
  3. Yi Wei1,
  4. Stephen J Pandol2,
  5. Aida Habtezion1
  1. 1 Division of Gastroenterology and Hepatology, Stanford University School of Medicine, Stanford, California, USA
  2. 2 Department of Veterans Affairs, Cedars-Sinai Medical Center, Los Angeles, California, USA
  1. Correspondence to Dr Aida Habtezion, Division of Gastroenterology and Hepatology, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA; aidah{at}stanford.edu

Abstract

Objective Chronic pancreatitis (CP) is an inflammatory disease with progressive fibrosis leading to exocrine and endocrine dysfunction. Currently, there are no approved effective therapies for CP. Stimulator of interferon genes (STING) signalling is a key innate immune sensor of DNA. In this study, we evaluated the role of STING signalling in CP.

Design We used an experimental model of CP to test the effect of STING signalling in STING wild-type and knockout mice as well as bone marrow chimaeras (BMCs). STING was activated using a pharmacological agent. Since we found changes in Th17 cells, we used neutralising and control antibodies to determine the role of IL-17A. The effect of STING signalling was further explored in IL-17A generation and we examined the effect of IL-17A on pancreatic stellate cells (PSCs). Human pancreas from patients with CP and without CP were also stained for IL-17A.

Results STING activation decreased CP-associated pancreatic inflammation and fibrosis, whereas absence of STING led to worsening of the disease. BMCs showed that leucocytes play an important role in STING signalling–mediated amelioration of experimental CP. STING deletion was associated with increased Th17 cell infiltration in the pancreas, whereas STING agonist limited this Th17 response. Importantly, anti-IL-17A antibody treatment mitigated the severity of CP in the absence of STING signalling. STING deficiency promoted Th17 polarisation and PSCs express functional IL-17 receptor by upregulating fibrosis genes. Compared with tumour margins, pancreas from patients with CP had significant increase in IL-17A+ cells.

Conclusion Unlike acute pancreatitis, STING activation is protective in CP. STING signalling is important in regulating adaptive immune responses by diminishing generation of IL-17A during CP and presents a novel therapeutic target for CP.

  • chronic pancreatitis
  • experimental pancreatitis
  • interleukins
View Full Text

Statistics from Altmetric.com

Footnotes

  • QZ and MM contributed equally.

  • Contributors QZ, MM and AH designed the experiment, analysed the data and wrote the manuscript. QZ, MM and YW performed the research. SJP provided key input. AH provided overall guide and supervision.

  • Funding This work was supported by the National Institute of Health (NIH) grants DK092421 and DK105264 (to AH), P01 DK098108 and R01 AA024464 (to SJP).

  • Competing interests None declared.

  • Ethics approval Local ethics committee.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Correction notice This article has been corrected since it published Online First. The equal contribution statement has been added.

  • Patient consent for publication Obtained.

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.