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Original article
Fine needle aspirates comprehensively sample intrahepatic immunity
  1. Upkar S Gill1,
  2. Laura J Pallett2,
  3. Niclas Thomas2,
  4. Alice R Burton2,
  5. Amit A Patel3,
  6. Simon Yona3,
  7. Patrick T F Kennedy1,
  8. Mala K Maini2
  1. 1 Barts Liver Centre, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK
  2. 2 Division of Infection and Immunity, Institute of Immunity and Transplantation, University College London, London, UK
  3. 3 Division of Medicine, University College London, London, UK
  1. Correspondence to Professor Mala K Maini, Division of Infection and Immunity, Institute of Immunity and Transplantation, UCL, London, UK; m.maini{at}ucl.ac.uk

Abstract

Objective In order to refine new therapeutic strategies in the pipeline for HBV cure, evaluation of virological and immunological changes compartmentalised at the site of infection will be required. We therefore investigated if liver fine needle aspirates (FNAs) could comprehensively sample the local immune landscape in parallel with viable hepatocytes.

Design Matched blood, liver biopsy and FNAs from 28 patients with HBV and 15 without viral infection were analysed using 16-colour multiparameter flow cytometry.

Results The proportion of CD4 T, CD8 T, Mucosal Associated Invariant T cell (MAIT), Natural Killer (NK) and B cells identified by FNA correlated with that in liver biopsies from the same donors. Populations of Programmed Death-1 (PD-1)hiCD39hi tissue-resident memory CD8 T cells (CD69+CD103+) and liver-resident NK cells (CXCR6+T-betloEomeshi), were identified by both FNA and liver biopsy, and not seen in the blood. Crucially, HBV-specific T cells could be identified by FNAs at similar frequencies to biopsies and enriched compared with blood. FNAs could simultaneously identify populations of myeloid cells and live hepatocytes expressing albumin, Scavenger Receptor class B type 1 (SR-B1), Programmed Death-Ligand 1 (PD-L1), whereas hepatocytes were poorly viable after the processing required for liver biopsies.

Conclusion We demonstrate for the first time that FNAs identify a range of intrahepatic immune cells including locally resident sentinel HBV-specific T cells and NK cells, together with PD-L1-expressing hepatocytes. In addition, we provide a scoring tool to estimate the extent to which an individual FNA has reliably sampled intrahepatic populations rather than contaminating blood. The broad profiling achieved by this less invasive, rapid technique makes it suitable for longitudinal monitoring of the liver to optimise new therapies for HBV.

  • fine needle aspirate
  • liver biopsy
  • hepatitis B virus
  • tissue-resident immunity
  • hepatocytes
  • intrahepatic-immune monitoring
  • HBV-specific T cells

This is an open access article distributed in accordance with the Creative Commons Attribution 4.0 Unported (CC BY 4.0) license, which permits others to copy, redistribute, remix, transform and build upon this work for any purpose, provided the original work is properly cited, a link to the licence is given, and indication of whether changes were made. See: https://creativecommons.org/licenses/by/4.0/.

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Footnotes

  • PTFK and MKM are joint senior authors.

  • USG and LJP contributed equally.

  • PTFK and MKM contributed equally.

  • Contributors Study concept and design: USG, LJP, PTFK, MKM; Acquisition of data: USG, LJP, ARB; Analysis and interpretation of data: USG, LJP, NT, AAP, SY, MKM; Mathematical and statistical analysis: USG, LJP, NT; Obtained funding: USG, PTFK, MKM; Administrative/technical/material/ethics support: USG, PTFK, MKM; Study supervision: PTFK, MKM; Drafting of manuscript: USG, LJP, MKM; Critical revision of manuscript: USG, LJP, NT, ARB, AAP, SY, PTFK, MKM.

  • Funding This work was supported by a Wellcome Trust Clinical Research Training Fellowship (107389/Z/15/Z) awarded to USG, a Barts and The London Charity Project Grant (723/1795) and an NIHR Research for patient benefit award (PB-PG-0614-34087) to PTFK, a Medical Research Council grant (G0801213) and a Wellcome Trust Senior Investigator Award and Enhancement (101849/Z/13/A) to MKM.

  • Competing interests USG, NT, ARB, AAP, SY have no conflicts of interest to declare; LJP has participated in a Gilead advisory board; PTFK has collaborative grant funding from Gilead, participates in advisory board/provides consultancy to Gilead, Janssen and is an investigator for industry-led trials with Gilead, Janssen, Alere, Assembly Biosciences. MKM’s laboratory has collaborative grant funding from Gilead, Roche and Immunocore; MKM participates in advisory boards/provides consultancy to Gilead, Roche, Arbutus Biopharma, Immunocore, Janssen.

  • Patient consent Not required.

  • Ethics approval The study was approved by the local research ethics committee (Brent Research Ethics committee, reference: 16/LO/1699) and complied with the Declaration of Helsinki .

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Correction notice This article has been corrected since it published Online First. The ’FNA tool' paragraphic has been updated.

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