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Original research
Novel scheme for non-invasive gut bioinformation acquisition with a magnetically controlled sampling capsule endoscope
  1. Zhen Ding1,
  2. Weijun Wang1,
  3. Kun Zhang1,
  4. Fanhua Ming2,
  5. Tianyi Yangdai2,
  6. Tao Xu1,
  7. Huiying Shi1,
  8. Yuhui Bao2,
  9. Hailing Yao1,
  10. Hangyu Peng2,
  11. Chaoqun Han1,
  12. Weiwei Jiang1,
  13. Jun Liu1,
  14. Xiaohua Hou1,
  15. Rong Lin1
  1. 1 Division of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
  2. 2 R&D department, ANKON Technologies, Wuhan, China
  1. Correspondence to Dr Rong Lin, Department of Gastroenterology, Wuhan Union Hospital, Wuhan 430022, China; selinalin35{at}hotmail.com

Abstract

Objective Intestinal flora and metabolites are associated with multiple systemic diseases. Current approaches for acquiring information regarding microbiota/metabolites have limitations. We aimed to develop a precise magnetically controlled sampling capsule endoscope (MSCE) for the convenient, non-invasive and accurate acquisition of digestive bioinformation for disease diagnosis and evaluation.

Design The MSCE and surgery were both used for sampling both jejunal and ileal GI content in the control and antibiotic-induced diarrhoea groups. The GI content was then used for microbiome profiling and metabolomics profiling.

Results Compared with surgery, our data showed that the MSCE precisely acquired data regarding the intestinal flora and metabolites, which was effectively differentiated in different intestinal regions and disease models. Using MSCE, we detected a dramatic decrease in the abundance of Bacteroidetes, Patescibacteria and Actinobacteria and hippuric acid levels, as well as an increase in the abundance of Escherichia–Shigella and the 2-pyrrolidinone levels were detected in the antibiotic-induced diarrhoea model by MSCE. MSCE-mediated sampling revealed specific gut microbiota/metabolites including Enterococcus, Lachnospiraceae, acetyl-L-carnitine and succinic acid, which are related to metabolic diseases, cancers and nervous system disorders. Additionally, the MSCE exhibited good sealing characteristics with no contamination after sampling.

Conclusions We present a newly developed MSCE that can non-invasively and accurately acquire intestinal bioinformation via direct visualization under magnetic control, which may further aid in disease prevention, diagnosis, prognosis and treatment.

  • gastrointesinal endoscopy
  • intestinal bacteria
  • inflammatory bowel disease

Data availability statement

Data are available upon reasonable request.

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Data availability statement

Data are available upon reasonable request.

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Footnotes

  • ZD, WW and KZ contributed equally.

  • Correction notice This article has been corrected since it published Online First. The provenance and peer review statement has been included.

  • Contributors RL, XH, ZD, WJW and KZ designed the study. WW, KZ, FM, TY, TX and HY did the experiment and collected the samples. FM, TY, YB and HP contributed to operate the machine. HS, CH, WJ and JL interpreted the data and analyses. WW and KZ wrote the first draft of the manuscript, and all authors reviewed, contributed to, and approved the manuscript.

  • Funding This study was supported by the National Natural Science Foundation of China (Nos. 81770539, 81330014, 81572428, 81272656, 81974068 and 81900580), and the National Key Research and Development Program of China (No. 2017YFC0110003).

  • Competing interests None declared.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Supplemental material This content has been supplied by the author(s). It has not been vetted by BMJ Publishing Group Limited (BMJ) and may not have been peer-reviewed. Any opinions or recommendations discussed are solely those of the author(s) and are not endorsed by BMJ. BMJ disclaims all liability and responsibility arising from any reliance placed on the content. Where the content includes any translated material, BMJ does not warrant the accuracy and reliability of the translations (including but not limited to local regulations, clinical guidelines, terminology, drug names and drug dosages), and is not responsible for any error and/or omissions arising from translation and adaptation or otherwise.

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