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Abstract
Background This study aimed to develop an efficient method for preparing sterile supernatant from human feces and to investigate the metagenomic and metabolomic profiles of the supernatant, as well as the potential of the method in removing pro-inflammatory metabolites.
Methods We established an innovative filtration and concentration process for extracting sterile supernatant from human feces. This process was designed to preserve microbial components such as bacteriophages in the supernatant while eliminating potentially harmful metabolites. Deep analyses of the prepared sterile supernatant were conducted using metagenomics and metabolomics techniques.
Results Metagenomic analysis revealed a rich metagenome in the sterile supernatant, including various bacteriophages that may play significant roles in maintaining gut microbial balance (IDDF2024-ABS-0264 Figure 1). Annotation and predictive analysis of antibiotic resistance genes indicated that our preparation process could reduce the abundance of drug-resistant genes (IDDF2024-ABS-0264 Figure 2, IDDF2024-ABS-0264 Figure 3). Metabolomic data indicated that we successfully removed multiple known pro-inflammatory metabolites, such as certain lipopolysaccharides and inflammatory mediators, suggesting the process has potential value in improving the gut environment (IDDF2024-ABS-0264 Figure 4, IDDF2024-ABS-0264 Figure 5, IDDF2024-ABS-0264 Figure 6).
Conclusions This study provides an effective method for preparing sterile supernatant from human feces and unveils its unique metagenomic and metabolomic characteristics. Our method demonstrates significant efficacy in removing pro-inflammatory metabolites, offering technical support for future utilization of fecal sterile supernatant in gut microbiota modulation and the treatment of inflammatory-related diseases.