Article Text

other Versions

Download PDFPDF
Original article
Imbalance of the renin–angiotensin system may contribute to inflammation and fibrosis in IBD: novel therapeutic target?
  1. Mayur Garg1,2,
  2. Simon G Royce3,
  3. Chris Tikellis4,
  4. Claire Shallue1,
  5. Duygu Batu4,
  6. Elena Velkoska5,
  7. Louise M Burrell5,
  8. Sheila K Patel5,
  9. Lauren Beswick1,
  10. Anvesh Jackson1,
  11. Kaushali Britto6,
  12. Matthew Lukies1,
  13. Pavel Sluka1,
  14. Hady Wardan1,
  15. Yumiko Hirokawa7,
  16. Chin Wee Tan7,
  17. Maree Faux7,
  18. Antony W Burgess7,8,
  19. Patrick Hosking9,
  20. Shaun Monagle9,
  21. Merlin Thomas4,
  22. Peter R Gibson6,
  23. John Lubel1
  1. 1 Eastern Health Clinical School, Monash University, Box Hill, Victoria, Australia
  2. 2 Gastroenterology and Hepatology, Royal Melbourne Hospital, Melbourne, Victoria, Australia
  3. 3 Medicine, Monash University Central Clinical School, Melbourne, Victoria, Australia
  4. 4 Diabetes, Monash University Central Clinical School, Melbourne, Victoria, Australia
  5. 5 Department of Medicine, University of Melbourne, Heidelberg, Victoria, Australia
  6. 6 Gastroenterology, Alfred Health, Melbourne, Victoria, Australia
  7. 7 Structural Biology, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
  8. 8 Medical Biology, University of Melbourne, Melbourne, Victoria, Australia
  9. 9 Pathology, Box Hill Hospital, Eastern Health, Box Hill, Victoria, Australia
  1. Correspondence to Dr Mayur Garg, Eastern Health Clinical School, Monash University, Box Hill, VIC 3800, Australia; mayur.garg{at}monash.edu

Footnotes

  • Contributors All authors approved the final version. Each author’s contribution is as follows:

    MG conceived and designed the studies; performed specimen and data collection and experiments including PCR, immunohistochemistry, serum ACE2 activity analyses; analysed and interpreted the data; and wrote the manuscript.

    SGR designed and performed immunohistochemistry studies and cell culture studies, analysed and interpreted the data, critically appraised the manuscript and approved the final version.

    CT designed and performed PCR and tissue ACE2 activity studies, analysed and interpreted the data, critically appraised the manuscript and approved the final version.

    CS performed PCR studies, critically appraised the manuscript and approved the final version.

    DB performed tissue ACE2 activity assays, critically appraised the manuscript and approved the final version.

    EV performed plasma ACE2 activity assays, critically appraised the manuscript and approved the final version.

    LMB designed and supervised plasma ACE2 activity assays, critically appraised the manuscript and approved the final version.

    LB collected patient data for retrospective cohort and case–control studies and approved the final version.

    AJ collected patient data for retrospective cohort and case–control studies and approved the final version.

    KB collected patient data for retrospective cohort and case–control studies, and approved the final version.

    ML collected patient data for retrospective cohort and case–control studies and approved the final version.

    PS performed and assisted with the PCR studies, critically appraised the manuscript and approved the final version.

    HW performed and assisted with the PCR studies, critically appraised the manuscript and approved the final version.

    YH isolated colonic tissue for cell culture studies, critically appraised the manuscript and approved the final version.

    CWT isolated colonic tissue for cell culture studies, critically appraised the manuscript and approved the final version.

    MF isolated colonic tissue for cell culture studies, critically appraised the manuscript and approved the final version.

    AWB designed and supervised colonic tissue isolation for cell culture studies, critically appraised the manuscript and approved the final version.

    PH analysed the histological activity of colonic biopsies, critically appraised the manuscript and approved the final version.

    SM analysed the histological activity of colonic biopsies, critically appraised the manuscript and approved the final version.

    MT supervised PCR and tissue ACE2 activity studies, critically appraised the manuscript and approved the final version.

    PRG conceived, designed and supervised the studies, critically appraised the manuscript and approved the final version.

    JL conceived, designed and supervised the studies, analysed and interpreted the data, critically appraised the manuscript and approved the final version.

  • Funding statement This work was supported by the Gastroenterological Society of Australia Scholarship awarded to MG and a Broad Medical Research Program Grant awarded to JL.

  • Competing interests None declared.

  • Patient consent for publication Not required.

  • Ethics approval Written informed consent was obtained from all participants for the prospective studies where patient information and samples were collected. No informed consent was required from the patients who were part of retrospective cohort and case–control studies, in which anonymised patient data were collected. The protocols for these studies were approved by the Eastern Health Department of Research and Ethics (E03-1112 and LR93-1112).

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data availability statement The authors declare that most of the data supporting the findings of this study are available within the paper and its supplementary information files. All other data are available from the corresponding author upon reasonable request.

View Full Text

Statistics from Altmetric.com

Footnotes

  • Contributors All authors approved the final version. Each author’s contribution is as follows:

    MG conceived and designed the studies; performed specimen and data collection and experiments including PCR, immunohistochemistry, serum ACE2 activity analyses; analysed and interpreted the data; and wrote the manuscript.

    SGR designed and performed immunohistochemistry studies and cell culture studies, analysed and interpreted the data, critically appraised the manuscript and approved the final version.

    CT designed and performed PCR and tissue ACE2 activity studies, analysed and interpreted the data, critically appraised the manuscript and approved the final version.

    CS performed PCR studies, critically appraised the manuscript and approved the final version.

    DB performed tissue ACE2 activity assays, critically appraised the manuscript and approved the final version.

    EV performed plasma ACE2 activity assays, critically appraised the manuscript and approved the final version.

    LMB designed and supervised plasma ACE2 activity assays, critically appraised the manuscript and approved the final version.

    LB collected patient data for retrospective cohort and case–control studies and approved the final version.

    AJ collected patient data for retrospective cohort and case–control studies and approved the final version.

    KB collected patient data for retrospective cohort and case–control studies, and approved the final version.

    ML collected patient data for retrospective cohort and case–control studies and approved the final version.

    PS performed and assisted with the PCR studies, critically appraised the manuscript and approved the final version.

    HW performed and assisted with the PCR studies, critically appraised the manuscript and approved the final version.

    YH isolated colonic tissue for cell culture studies, critically appraised the manuscript and approved the final version.

    CWT isolated colonic tissue for cell culture studies, critically appraised the manuscript and approved the final version.

    MF isolated colonic tissue for cell culture studies, critically appraised the manuscript and approved the final version.

    AWB designed and supervised colonic tissue isolation for cell culture studies, critically appraised the manuscript and approved the final version.

    PH analysed the histological activity of colonic biopsies, critically appraised the manuscript and approved the final version.

    SM analysed the histological activity of colonic biopsies, critically appraised the manuscript and approved the final version.

    MT supervised PCR and tissue ACE2 activity studies, critically appraised the manuscript and approved the final version.

    PRG conceived, designed and supervised the studies, critically appraised the manuscript and approved the final version.

    JL conceived, designed and supervised the studies, analysed and interpreted the data, critically appraised the manuscript and approved the final version.

  • Funding statement This work was supported by the Gastroenterological Society of Australia Scholarship awarded to MG and a Broad Medical Research Program Grant awarded to JL.

  • Competing interests None declared.

  • Patient consent for publication Not required.

  • Ethics approval Written informed consent was obtained from all participants for the prospective studies where patient information and samples were collected. No informed consent was required from the patients who were part of retrospective cohort and case–control studies, in which anonymised patient data were collected. The protocols for these studies were approved by the Eastern Health Department of Research and Ethics (E03-1112 and LR93-1112).

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data availability statement The authors declare that most of the data supporting the findings of this study are available within the paper and its supplementary information files. All other data are available from the corresponding author upon reasonable request.

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.