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ZEB1 promotes inflammation and progression towards inflammation-driven carcinoma through repression of the DNA repair glycosylase MPG in epithelial cells

Footnotes

  • OdB, LS-M and MC contributed equally as co-first authors to the work.

  • 36 CN, NP-P, MCM-C and LS contributed equally as co-second authors to the work.

  • Contributors OdB, LS-M and MC performed most of the experimental work in the study. CN and LS assisted in some analyses of mRNA expression; NP-P and MCM-C carried out some immunostaining experiments. RdC performed the microbiota next-generation sequencing and its bioinformatics analysis. MJF-A assisted with the procurement of some of the human samples as well as with the pathology analyses of human and mouse tissue immunostaining. DSD, AC, JM, AS and DCD provided important materials and insights to the study. AP conceived and supervised the study, and wrote the manuscript. All authors provided critical comments to the manuscript.

  • Funding The different parts of this study were independently funded by grants to AP from Catalan Agency for Management of University and Research Grants (AGAUR) (2014-SGR-475 and 2017-SGR-1174), Ministry of Economy and Competitiveness (SAF2014-52874-R and SAF2017-84918-R, National Scientific and Technical Research and Innovation 2013–2016 Plan, which is co-financed by the European Regional Development Fund of the European Union Commission), Fundació La Marató de TV3 (201330.10) and Avon Foundation SAU (AFSAU). The IDIBAPS Institute is partly funded by the CERCA Programme of Generalitat de Catalunya. OdB was subsequently supported by a fellowship from the Spanish Ministry of Education, Culture and Sports (MECS) (FPU programme, AP2010-5489) and by grants 2014-SGR-475, 201330.10 and AFSAU. LS-M is recipient of a PhD scholarship from MECS (FPU Programme, FPU14/06217). MC is supported by grants 201330.10 and SAF2014-52874-R to AP. CN was recipient of a PhD scholarship from AGAUR (AGAUR-2015-FI_B-00121) and later supported by grant 2017-SGR-1174 to AP. NPP and MCMC are recipients of PhD scholarships from the Spanish Ministry of Economy and Competitiveness (FPI Programme, BES-2015-073163 and BES-2015-075757, respectively). LS was supported by grants 201330.10, AVFSAU and 2017-SGR-1174 to AP.

  • Competing interests None declared.

  • Ethics approval The use of human samples was approved by the Clinical Ethics Research Committee at the Hospital Clinic of Barcelona under reference HCB/2018/0157. The use of mouse models followed the guidelines of the Animal Experimentation Ethics Committee at the University of Barcelona (Spain) and was approved under reference UB/301/16 and UB/302/16.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Correction notice This article has been corrected since it published Online First. The text in the abstract has been amended.

  • Patient consent for publication Obtained.

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Footnotes

  • OdB, LS-M and MC contributed equally as co-first authors to the work.

  • 36 CN, NP-P, MCM-C and LS contributed equally as co-second authors to the work.

  • Contributors OdB, LS-M and MC performed most of the experimental work in the study. CN and LS assisted in some analyses of mRNA expression; NP-P and MCM-C carried out some immunostaining experiments. RdC performed the microbiota next-generation sequencing and its bioinformatics analysis. MJF-A assisted with the procurement of some of the human samples as well as with the pathology analyses of human and mouse tissue immunostaining. DSD, AC, JM, AS and DCD provided important materials and insights to the study. AP conceived and supervised the study, and wrote the manuscript. All authors provided critical comments to the manuscript.

  • Funding The different parts of this study were independently funded by grants to AP from Catalan Agency for Management of University and Research Grants (AGAUR) (2014-SGR-475 and 2017-SGR-1174), Ministry of Economy and Competitiveness (SAF2014-52874-R and SAF2017-84918-R, National Scientific and Technical Research and Innovation 2013–2016 Plan, which is co-financed by the European Regional Development Fund of the European Union Commission), Fundació La Marató de TV3 (201330.10) and Avon Foundation SAU (AFSAU). The IDIBAPS Institute is partly funded by the CERCA Programme of Generalitat de Catalunya. OdB was subsequently supported by a fellowship from the Spanish Ministry of Education, Culture and Sports (MECS) (FPU programme, AP2010-5489) and by grants 2014-SGR-475, 201330.10 and AFSAU. LS-M is recipient of a PhD scholarship from MECS (FPU Programme, FPU14/06217). MC is supported by grants 201330.10 and SAF2014-52874-R to AP. CN was recipient of a PhD scholarship from AGAUR (AGAUR-2015-FI_B-00121) and later supported by grant 2017-SGR-1174 to AP. NPP and MCMC are recipients of PhD scholarships from the Spanish Ministry of Economy and Competitiveness (FPI Programme, BES-2015-073163 and BES-2015-075757, respectively). LS was supported by grants 201330.10, AVFSAU and 2017-SGR-1174 to AP.

  • Competing interests None declared.

  • Ethics approval The use of human samples was approved by the Clinical Ethics Research Committee at the Hospital Clinic of Barcelona under reference HCB/2018/0157. The use of mouse models followed the guidelines of the Animal Experimentation Ethics Committee at the University of Barcelona (Spain) and was approved under reference UB/301/16 and UB/302/16.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Correction notice This article has been corrected since it published Online First. The text in the abstract has been amended.

  • Patient consent for publication Obtained.

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