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The colonoscopic leakage model: a new model to study the intestinal wound healing at molecular level
  1. Radu Bachmann1,2,
  2. Matthias Van Hul2,
  3. Daniel Leonard1,
  4. Nathalie M Delzenne2,
  5. Alex Kartheuser1,
  6. Patrice D Cani2
  1. 1Colorectal Surgery Unit, Cliniques universitaires Saint-Luc, UCLouvain, Universite catholique de Louvain, Brussels, Belgium
  2. 2Metabolism and Nutrition Research Group, Louvain Drug Research Institute, Walloon Excellence in Life Sciences and BIOtechnology (WELBIO), UCLouvain, Université catholique de Louvain, Brussels, Belgium
  1. Correspondence to Professor Patrice D Cani, Louvain Drug Research Institute, UCLouvain, Universite catholique de Louvain, WELBIO, Metabolism and Nutrition, Avenue E. Mounier, 73 B1.73.11 B-1200, Brussels, Belgium; patrice.cani{at}uclouvain.be

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We describe a novel, fast-track, innovative intestinal wound healing model developed in mice called the ‘colonoscopic leakage model’. This model uses a colonoscopic biopsy grasper to make a controlled transmural perforation in the colon. Similar to other classic anastomotic leakage models, we observed about 50% incidence of peritonitis, although this model is less invasive, faster and avoids intestinal occlusion as well as massive bodyweight loss. Therefore, this preclinical model could be used to test potential therapeutic agents and to investigate the different molecular wound healing mechanisms.

In more detail

Despite significant progress in the medical field, the mechanisms of healing of a wound in the GI tract are still not completely identified, which limits the options for positive interventions at the local level to support the healing processes.

Even though many influencing factors have been studied, it is very challenging to acquire knowledge of the local pathophysiology and to study the impact of different pharmacological and microbiological conditions at the molecular level. To overcome these difficulties, the use of experimental models that help in understanding the local conditions has become indispensable.

The models proposed in the literature to study the mechanisms of intestinal wound healing are mainly anastomotic leakage models. Among them, the one proposed by Pommergaard et al1 is the most commonly used technique (online supplementary video 1). This rodent model is based on the variation of the number of intestinal anastomotic sutures after segmental colonic resection (5 vs 8 stitches) in order to achieve a probability of 50% of anastomotic leakage (figure 1A and online supplementary videos 2–4) and peritonitis in the anastomotic leakage group.

Supplementary video

[gutjnl-2020-321234supp001.mov]

Supplementary video

[gutjnl-2020-321234supp002.mov]

Supplementary video

[gutjnl-2020-321234supp003.mov]

Supplementary video

[gutjnl-2020-321234supp004.mov]
Figure 1

Anastomotic leakage model. (A) The anastomotic leakage model is feasible with an anastomotic leakage and …

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