Full-field optical coherence tomography (FFOCT) is an imaging technique of biological tissue based on tissue light reflectance analysis. We evaluated the feasibility of imaging fresh digestive mucosal biopsies after a quick mounting procedure (5 min) using two distinct modalities of FFOCT. In static FFOCT mode, we gained high-resolution images of general gut tissue-specific architecture, such as oesophageal papillae, gastric pits, duodenal villi and colonic crypts. In dynamic FFOCT mode, we imaged individual epithelial cells of the mucosal lining with a cellular or subcellular resolution and identified cellular components of the lamina propria. FFOCT represents a promising dye-free imaging tool for on-site analysis of gut tissue remodelling.
- epithelial barrier
- gastrointesinal endoscopy
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LQ and RO are joint first authors.
MN and EC are joint senior authors.
MN and EC contributed equally.
Contributors Conceptualisation: LQ, RO, MN and EC. Endoscopic examinations and biopsy collection: EC. Pathology review: JFM and CBos. Data curation: LQ, RO, MJG, EBG and CBr. Formal analysis: LQ and MN. Funding acquisition: LQ, MN and EC. Investigation: LQ, RO, JFM, CBos and CBr. Supervision: MN and EC. Writing the original draft: LQ, RO, MJG and MN. Writing, review and editing: LQ, MN, EC, CBoc, CBr and MJG.
Funding Grant for equipment funding: Plan Cancer Inserm 2016, Mibiogate project by the region Pays de la Loire, DHU Oncogreffe, CEREDI: Centre de Recherche en Endoscopie Digestive.
Competing interests CBoc is one of the founders of the company LLTech and holds shares in this company. EBG is employed by LLTech.
Patient and public involvement Patients and/or the public were not involved in the design, or conduct, or reporting, or dissemination plans of this research.
Patient consent for publication Not required.
Provenance and peer review Not commissioned; internally peer reviewed.
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