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In their outstanding study, Wei et al1 compared the intestinal microbiota (IM) of well-defined autoimmune hepatitis (AIH) patients to healthy controls. They reported significantly reduced IM alpha diversity and disparate IM compositional heterogeneity (beta diversity) with enrichment of Streptococcus, Veillonella, Klebsiella and Lactobacillus in AIH. The excellent achievement of this study is to have characterised the IM of untreated patients with AIH. However, differentiation between IM changes caused by AIH in particular and by chronic liver disease (CLD) in general was not possible because this study lacked a control group with CLD other than AIH.
In a pilot study, we analysed the IM of patients with CLD of mixed aetiology in comparison to healthy controls to clarify alterations across different stages of CLD (table 1 and online supplementary data). In contrast to the study by Wei et al, patients with recent intake of antibiotics were excluded. Additionally, only patients on proton pump inhibitors (PPIs), which are frequently taken by patients with CLD, were included to avoid a bias due to mixed PPI use, because PPI can alter the IM, in particular, the abundance of Veillonellaceae and Streptococcaceae.2 Based on investigations of normalised relative reads (n-RR) per IM taxon as well as IM alpha-diversity and beta-diversity analyses, we detected Veillonella …
JN, IB-D and US are joint senior authors.
Contributors Concept and design: FG, JN, IB-D and US; collection of patient-derived samples: FG, PLL and DJK; establishment of procedures/protocols and performance of experiments: FG, BK, CF, HDN, RG and MP; analysis of data: FG, PM, TRL and US; interpretation and integration of data: FG, PM, TRL, AMcH, JN, IB-D and US; writing of the manuscript: FG, PLL, JN, IB-D and US; supervision: AMcH, CS, AH, JN, IB-D and US; all authors proofread the final version of the manuscript.
Funding FG received a 'clinical leave stipend' by the German Center for Infection Research between August 2014 and February 2016.
Competing interests None declared.
Patient and public involvement Patients and/or the public were not involved in the design, conduct, reporting or dissemination plans of this research.
Patient consent for publication Not required.
Provenance and peer review Not commissioned; externally peer reviewed.
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