Article Text

Download PDFPDF
Original research
Long-term serological, virological and histological responses to RNA inhibition by ARC-520 in Chinese chronic hepatitis B patients on entecavir treatment
  1. Man-Fung Yuen1,
  2. Danny Ka-Ho Wong1,
  3. Thomas Schluep2,
  4. Ching-Lung Lai1,
  5. Carlo Ferrari3,
  6. Stephen Locarnini4,
  7. Regina Cheuk-Lam Lo5,6,
  8. Robert G Gish7,
  9. James Hamilton2,
  10. Christine I Wooddell8,
  11. Lung Yi Mak1,
  12. Bruce D Given2
  1. 1Department of Medicine, The University of Hong Kong, Hong Kong, China
  2. 2Arrowhead Pharmaceuticals, Pasadena, California, USA
  3. 3Unit of Infectious Diseases and Hepatology, University of Parma, Parma, Italy
  4. 4Victorian Infectious Diseases Reference Laboratory, Melbourne, Victoria, Australia
  5. 5The University of Hong Kong, Hong Kong, Hong Kong
  6. 6Department of Pathology, The University of Hong Kong, Hong Kong, China
  7. 7Hepatitis B Foundation, Doylestown, PA, USA
  8. 8Arrowhead Pharmaceuticals, Madison, Wisconsin, USA
  1. Correspondence to Professor Man-Fung Yuen, Department of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong, China; mfyuen{at}hkucc.hku.hk

Abstract

Objective We examined the serological, virological (in serum and liver) and histological profiles in chronic hepatitis B virus (HBV) patients during and after completion of multiple dose (MD) ARC-520.

Design The present phase 1b study was a multidose, open-label extension cohort of patients that had received single dose ARC-520 in our previous study. Eight patients received 4–9 4 weekly doses of MD ARC-520 and entecavir. Liver biopsies were performed in six patients. Intrahepatic and serum HBV DNA, HBV RNA and viral antigens were measured.

Results All patients had 28.9–30.4 months of follow-up after the last MD. All three hepatitis B e antigen (HBeAg)-positive patients had profound reductions in hepatitis B surface antigen (HBsAg), HBeAg, hepatitis B core-related antigen and HBV RNA with two undergoing HBeAg seroconversion. One further achieved HBsAg seroconversion (anti-HBs level of 25.1 IU/L) and the remaining two had HBsAg reductions of −1.7 and −3.5 log IU/mL >30 months after MD. Among the five HBeAg-negative patients, four had modest HBsAg reduction >29 months after completion of MD and one achieved HBsAg seroconversion (anti-HBs level of 152.5 IU/L) and was negative for liver HBsAg staining. Entecavir was successfully stopped in this patient 12 months after HBsAg seroconversion. Temporally related alanine aminotransferase elevations preceded by HBsAg reductions were observed in three patients suggesting immune activation. HBcAg staining was negative in all six biopsied patients. Two patients with <10% HBsAg positive staining of hepatocytes had correspondingly low serum HBsAg levels of 1.5 and 11.5 IU/mL.

Conclusions MD ARC-520 therapy achieved sustained and profound reductions of viral antigens and HBV RNA. HBsAg seroclearance was achievable.

Trial registration number NCT02065336.

  • hepatitis B

Statistics from Altmetric.com

Footnotes

  • M-FY and DK-HW contributed equally.

  • Contributors M-FY, BDG, JH, RC-LL, C-LL and L-YM performed the clinical trial; M-FY, DK-HW, TS, JH, CIW and BDG analysed the data; M-FY, TS, JH, RGG, BDG and CIW designed the studies; M-FY, DK-HW, CIW and BDG drafted the paper; M-FY, DK-HW, TS, CF, SAL, RC-LL, RGG, C-LL, JH, CIW, LYM and BDG critically revised the paper. All authors had access to the study data and had reviewed and approved the final manuscript, including the author list.

  • Funding This study was funded in full by Arrowhead Pharmaceutical. Award/grant number: not applicable.

  • Competing interests M-FY is advisory board member and received speaker’s fees from AbbVie, Janssen, Biocartis NV, Bristol Myers Squibb, Fujirebio Incorporation, Gilead Sciences, Merck Sharp and Dohme, Sysmex Corporation. M-FY does consulting for Aligos Pharmaceuticals, Assembly Biosciences, Arbutus Biopharma, Dicerna Pharmaceuticals, Clear-B Therapeutics, GlaxoSmithKline, Immunocore, Springbank Pharmaceuticals and also received research funding from Bristol Myers Squibb and Gilead Sciences. TS, JH, BDG, and CIW are employed by Arrowhead Pharmaceuticals and may hold stock. SL does consulting for Arrowhead Pharmaceuticals, Assembly Biosciences, Aligos Pharmaceuticals, Clear-B Therapeutics and GlaxoSmithKline. C-LL is on the Advisory Committees or Review Panels of: Bristol-Myers Squibb and Gilead Sciences. C-LL does consulting for Arrowhead Pharmaceuticals, Bristol-Myers Squibb, and Gilead Sciences. C-LL is on the Speaker’s Bureau for: Bristol-Myers Squibb and Gilead Sciences. RGG has had Grants/Research Support from Gilead Sciences and Merck & Co. RGG has performed as consultant and/or advisor to Akshaya Pharmaceuticals, Arbutus Biopharma, Arrowhead Pharmaceuticals, Bristol-Myers Squibb, ContraVir Pharmaceuticals, Enyo Pharma, Gilead Sciences, HumAbs BioMed, Ionis Pharmaceuticals, Merck & Co., Nanogen Biopharmaceutical and Novira Therapeutics. RGG has current activity with the Scientific or Clinical Advisory Boards of Arrowhead Pharmaceuticals, Merck & Co, ContraVir Pharmaceuticals, Gilead Sciences, Isis Pharmaceuticals, Enyo Pharma, HumAbs BioMed and Nanogen Biopharmaceutical. RGG is a member of the Speakers Bureau for Bristol-Myers Squibb, Gilead Sciences, and Merck & Co. RGG has stock options with Arrowhead Pharmaceuticals.

  • Patient consent for publication Not required.

  • Ethics approval Both the initial multicentre study and the present single-centre extension study were approved by the Institution Review Board (IRB) of the University of Hong Kong and Hospital Authority Hong Kong West Cluster, Hong Kong (UW 13–542). They were conducted in accordance with the 2008 Declaration of Helsinki and good clinical practice guidelines.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data availability statement All data relevant to the study are included in the article or uploaded as online supplemental information.

  • Supplemental material This content has been supplied by the author(s). It has not been vetted by BMJ Publishing Group Limited (BMJ) and may not have been peer-reviewed. Any opinions or recommendations discussed are solely those of the author(s) and are not endorsed by BMJ. BMJ disclaims all liability and responsibility arising from any reliance placed on the content. Where the content includes any translated material, BMJ does not warrant the accuracy and reliability of the translations (including but not limited to local regulations, clinical guidelines, terminology, drug names and drug dosages), and is not responsible for any error and/or omissions arising from translation and adaptation or otherwise.

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.