RT Journal Article
SR Electronic
T1 Leucocyte migration inhibition test in coeliac disease - a reappraisal.
JF Gut
JO Gut
FD BMJ Publishing Group Ltd and British Society of Gastroenterology
SP 311
OP 317
DO 10.1136/gut.24.4.311
VO 24
IS 4
A1 F G Simpson
A1 H P Field
A1 P D Howdle
A1 D A Robertson
A1 M S Losowsky
YR 1983
UL http://gut.bmj.com/content/24/4/311.abstract
AB Results of the direct leucocyte migration inhibition (LMI) test using gluten fraction III as antigen were unaffected by incorporation of puromycin into the culture medium at concentrations shown to prevent lymphokine mediated inhibition. Results of the LMI test performed with purified polymorphs were similar to and correlated with results of the standard LMI test using mixed leucocytes in both coeliacs and controls. The addition of purified T lymphocytes did not increase migration inhibition. Normal leucocytes incubated with serum from coeliac patients and washed showed marked migration inhibition when incubated with gluten fraction III. This sensitisation of normal leucocytes was prevented by preincubation with aggregated human IgG. These results suggest that leucocyte migration inhibition by gluten in coeliac disease is not due to lymphokine production by sensitised lymphocytes but is caused by cytophilic antibody.