RT Journal Article SR Electronic T1 Enumeration of lymphocyte populations defined by surface markers in the whole blood of patients with Crohn's disease. JF Gut JO Gut FD BMJ Publishing Group Ltd and British Society of Gastroenterology SP 766 OP 769 DO 10.1136/gut.23.9.766 VO 23 IS 9 A1 Pepys, E O A1 Fagan, E A A1 Tennent, G A A1 Chadwick, V S A1 Pepys, M B YR 1982 UL http://gut.bmj.com/content/23/9/766.abstract AB The proportions and absolute numbers of different lymphocyte populations were determined using alkaline phosphatase-labelled reagents in the whole peripheral blood of 22 patients with Crohn's disease. Monoclonal mouse anti-T cell antibody (OKT3) was used to identify T cells, polyvalent F(ab')2 anti-human immunoglobulin for B cells, C3b for C3b-receptor bearing cells, and soluble IgG antibody-antigen complexes for Fc(gamma)-receptor bearing cells. Endogenous myeloperoxidase served to distinguish monocytes. Application of this methodology to whole blood avoids the inevitable loss of cells which accompanies separation of mononuclear cells from blood and therefore permits precise enumeration of lymphocyte populations in the circulation. No significant difference from healthy adult controls was observed in any of the lymphocyte subsets tested.