PT  - JOURNAL ARTICLE
AU  - Al-Aghbar, M N
AU  - Alexander, G J
AU  - Nouri-Aria, K T
AU  - Neuberger, J
AU  - Eddleston, A L
AU  - Williams, R
TI  - In vitro effect of cyclosporin A on immunoglobulin production and concanavalin A induced suppression in primary biliary cirrhosis.
AID  - 10.1136/gut.27.3.317
DP  - 1986 Mar 01
TA  - Gut
PG  - 317--323
VI  - 27
IP  - 3
4099  - http://gut.bmj.com/content/27/3/317.short
4100  - http://gut.bmj.com/content/27/3/317.full
SO  - Gut1986 Mar 01; 27
AB  - The in vitro effect of Cyclosporin A on the regulation of immunoglobulin production was investigated in 16 patients with primary biliary cirrhosis. A significant improvement in concanavalin A induced suppression of IgG and IgM producing cells was observed after prior incubation of mononuclear cells with 300 ng/ml Cyclosporin A for 30 minutes. No effect was seen on spontaneous or pokeweed mitogen induced immunoglobulin production, nor on con A induced suppression if Cyclosporin A was added after 24 hours. Incubation of mononuclear cells with a variable dose of Cyclosporin A showed an effect only at 250-500 ng/ml. Higher and lower doses had no effect. This dose dependent effect of Cyclosporin A is likely to be related to a differential inhibitory effect on T helper and T suppressor cells and may underlie the clinical benefit being observed in current clinical trials.