TY - JOUR T1 - Helicobacter pylori secretes a chemotactic factor for monocytes and neutrophils. JF - Gut JO - Gut SP - 1020 LP - 1023 DO - 10.1136/gut.33.8.1020 VL - 33 IS - 8 AU - P M Craig AU - M C Territo AU - W E Karnes AU - J H Walsh Y1 - 1992/08/01 UR - http://gut.bmj.com/content/33/8/1020.abstract N2 - Helicobacter pylori is associated with an inflammatory reaction in the stomach and duodenum, yet the mechanism of this inflammatory infiltrate is unknown. The ability of Helicobacter pylori to secrete a factor that attracts leucocytes is investigated. Helicobacter pylori conditioned supernatant attracted neutrophils and monocytes with 50-100% of the activity of control chemotactic factor, 10(-8) M formyl-methionine-leucine-phenylalanine. Strains derived from individuals with ulcer or non-ulcer associated H pylori infections displayed similar chemotactic activity. Preliminary characterisation shows that the factor has a molecular weight of less than 3000, is heat stable, is acid resistant, and can be diluted at least 10-fold. Checkerboard analysis confirmed that the activity was chemotactic rather than chemokinetic. This chemotactic activity could play a role in the pathogenesis of Helicobacter pylori gastritis. ER -