TY - JOUR T1 - Cytochrome P450 expression in oesophageal cancer. JF - Gut JO - Gut SP - 599 LP - 603 DO - 10.1136/gut.35.5.599 VL - 35 IS - 5 AU - G I Murray AU - D Shaw AU - R J Weaver AU - J A McKay AU - S W Ewen AU - W T Melvin AU - M D Burke Y1 - 1994/05/01 UR - http://gut.bmj.com/content/35/5/599.abstract N2 - The cytochrome P450 superfamily of enzymes play a central part in the metabolism of carcinogens and anti-cancer drugs. The expression, cellular localisation, and distribution of different forms of P450 and the functionally associated enzymes epoxide hydrolase and glutathione S-transferases have been investigated in oesophageal cancer and non-neoplastic oesophageal tissue using immunohistochemistry. Expression of the different enzymes was confined to epithelial cells in both non-neoplastic samples and tumour samples except the CYP3A was also identified in mast cells and glutathione S-transferase pi was present in chronic inflammatory cells. CYP1A was present in a small percentage of non-neoplastic samples but both CYP2C and CYP3A were absent. Epoxide hydrolase was present in half of the non-neoplastic samples and the different classes of glutathione S-transferase were present in a low number of samples. In carcinomas CYP1A, CYP3A, epoxide hydrolase, and glutathione S-transferase pi were expressed in at least 60% of samples. The expression of glutathione S-transferases alpha and mu were significantly less in adenocarcinoma compared with squamous carcinoma. ER -