PT  - JOURNAL ARTICLE
AU  - Livingstone, J I
AU  - Filipe, M I
AU  - Wastell, C
TI  - Expression of transforming growth factor alpha in experimental gastric carcinogenesis.
AID  - 10.1136/gut.35.5.604
DP  - 1994 May 01
TA  - Gut
PG  - 604--607
VI  - 35
IP  - 5
4099  - http://gut.bmj.com/content/35/5/604.short
4100  - http://gut.bmj.com/content/35/5/604.full
SO  - Gut1994 May 01; 35
AB  - The induction of adenocarcinomas in the glandular stomach of the adult male Wistar rat by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) was used as a model to study the expression of the growth promoting peptide, transforming growth factor alpha (TGF alpha), during experimental gastric carcinogenesis. TGF alpha was identified using the monoclonal antibody Ab-2 and standard immunohistochemistry, together with a semiquantitative assessment of the intensity of expression. Immunoreactivity was confined to the differentiated compartment of the mucosa while the carcinogen MNNG caused a significant increase in the intensity of TGF alpha expression (p < 0.01), after as little as 16 weeks' exposure. In experimental adenocarcinomas, a change to a previously undescribed pattern of perinuclear TGF alpha expression was found, which may represent the site of intense TGF alpha production in the Golgi apparatus after malignant transformation.