PT  - JOURNAL ARTICLE
AU  - T Ajiki
AU  - T Fujimori
AU  - H Onoyama
AU  - M Yamamoto
AU  - S Kitazawa
AU  - S Maeda
AU  - Y Saitoh
TI  - K-ras gene mutation in gall bladder carcinomas and dysplasia.
AID  - 10.1136/gut.38.3.426
DP  - 1996 Mar 01
TA  - Gut
PG  - 426--429
VI  - 38
IP  - 3
4099  - http://gut.bmj.com/content/38/3/426.short
4100  - http://gut.bmj.com/content/38/3/426.full
SO  - Gut1996 Mar 01; 38
AB  - Epithelial dysplasia of gall bladder is an important precancerous lesion of gall bladder carcinogenesis. To investigate the frequency of K-ras gene mutation in gall bladder carcinoma and dysplasia, K-ras codon 12 mutations were investigated by the polymerase chain reaction/restriction enzyme based method following direct sequencing. Mutation was detected in 59% (30 of 51) of gall bladder carcinomas, in 73% (8 of 11) of gall bladder dysplasia in gall stone cases, and in 0% of the normal gall bladder epithelium. There was, however, no correlation between K-ras mutation and clinicopathological factors of gall bladder carcinoma. K-ras gene mutation occurs even in gall bladder dysplasia at an incidence similar to that in carcinomas, suggesting that testing for K-ras gene mutation may prove useful as an adjunct to bile cytological or biopsy analysis.