RT Journal Article
SR Electronic
T1 The effect of epidermal growth factor on brush border surface area and function in the distal remnant following resection in the rabbit
JF Gut
JO Gut
FD BMJ Publishing Group Ltd and British Society of Gastroenterology
SP 26
OP 32
DO 10.1136/gut.44.1.26
VO 44
IS 1
A1 J A Hardin
A1 B Chung
A1 E V O’Loughlin
A1 D G Gall
YR 1999
UL http://gut.bmj.com/content/44/1/26.abstract
AB Background Epidermal growth factor (EGF) has been shown to increase intestinal absorptive surface area and transport function in normal animals.Aims To examine the effect of EGF on absorptive surface area and brush border membrane function in a model of massive small bowel resection.Methods New Zealand white rabbits were randomised into two groups: a resected group (60% proximal small bowel resection); and an unmanipulated control group. Distal remnant tissue was examined 10 and 21 days postsurgery. In separate experiments oral EGF (40 μg/kg/day) was administered to resected animals from days 3 to 8 and animals were studied on day 10.Results Ten days postsurgery brush border surface area and total absorptive surface area were significantly increased in remnant tissue while brush border membrane vesicle (BBMV) glucose uptake was significantly decreased compared with controls. By 21 days brush border surface area returned to control levels though BBMV glucose uptake remained depressed. EGF treatment induced a further increase in brush border surface area in remnant intestine but did not alter BBMV glucose uptake.Conclusions Surgical resection results in significant elevations in absorptive surface area coupled with a decrease in brush border membrane transport function distal to the site of anastomosis. EGF enhances glucose uptake in remnant intestine via recruitment of additional microvillus membrane into the brush border.BBMVbrush border membrane vesicleEGFepidermal growth factorBrdUbromodeoxyuridineTMA-DPH1-[4-(trimethylamino)phenyl]-6-phenylhexatrieneDMSOdimethyl sulphoxideRESresectedCONcontrolSGLTsodium glucose linked transporter