RT Journal Article
SR Electronic
T1 High mobility group (HMG) non-histone chromosomal proteins HMG1 and HMG2 are significant target antigens of perinuclear anti-neutrophil cytoplasmic antibodies in autoimmune hepatitis
JF Gut
JO Gut
FD BMJ Publishing Group Ltd and British Society of Gastroenterology
SP 867
OP 873
DO 10.1136/gut.44.6.867
VO 44
IS 6
A1 J Sobajima
A1 S Ozaki
A1 H Uesugi
A1 F Osakada
A1 M Inoue
A1 Y Fukuda
A1 H Shirakawa
A1 M Yoshida
A1 A Rokuhara
A1 H Imai
A1 K Kiyosawa
A1 K Nakao
YR 1999
UL http://gut.bmj.com/content/44/6/867.abstract
AB BACKGROUND High mobility group (HMG) non-histone chromosomal proteins HMG1 and HMG2 have been identified as novel antigens of perinuclear anti-neutrophil cytoplasmic antibodies (p-ANCAs), and the existence of anti-HMG1 and anti-HMG2 antibodies in a population of patients with ulcerative colitis has been reported.AIMS To investigate whether HMG1 and HMG2 are target antigens for p-ANCAs in autoimmune hepatitis (AIH).PATIENTS Serum samples from 28 patients with AIH, 44 patients with primary biliary cirrhosis (PBC), 27 patients with chronic hepatitis C, and 23 patients with chronic hepatitis B were tested.METHODS ANCAs were detected by routine indirect immunofluorescence (IIF). Anti-HMG1 and anti-HMG2 antibodies were assayed by enzyme linked immunosorbent assay.RESULTS p-ANCAs were detected in 89% (25/28) of patients with AIH, 36% (16/44) of patients with PBC, 11% (3/27) of patients with chronic hepatitis C, and 13% (3/23) of patients with chronic hepatitis B. Anti-HMG1 and/or anti-HMG2 antibodies were detected in 89% (25/28) of patients with AIH, 70% (31/44) with PBC, 26% (7/27) with chronic hepatitis C, and 9% (2/23) with chronic hepatitis B. In AIH, anti-HMG1 and/or anti-HMG2 antibodies were detected in 96% (24/25) of p-ANCA positive patients. The p-ANCA staining pattern detected by IIF using sera from patients with AIH disappeared or decreased in titre after preincubation with a mixture of HMG1/HMG2. The presence and titres of those antibodies in AIH correlated significantly with those of p-ANCA, but not with those of anti-nuclear antibody or anti-smooth muscle antibody.CONCLUSIONS HMG1 and HMG2 are significant target antigens of p-ANCA in AIH.ANCAanti-neutrophil cytoplasmic antibodyIIFindirect immunofluorescencec-ANCAcytoplasmic anti-neutrophil cytoplasmic antibodyp-ANCAperinuclear anti-neutrophil cytoplasmic antibodyAIHautoimmune hepatitisPSCprimary sclerosing cholangitisHMGhigh mobility groupELISAenzyme linked immunosorbent assayPBCprimary biliary cirrhosisANAanti-nuclear antibodySMAanti-smooth muscle antibodyAMAanti-mitochondrial antibody