PT  - JOURNAL ARTICLE
AU  - D Nehra
AU  - P Howell
AU  - C P Williams
AU  - J K Pye
AU  - J Beynon
TI  - Toxic bile acids in gastro-oesophageal reflux disease: influence of gastric acidity
AID  - 10.1136/gut.44.5.598
DP  - 1999 May 01
TA  - Gut
PG  - 598--602
VI  - 44
IP  - 5
4099  - http://gut.bmj.com/content/44/5/598.short
4100  - http://gut.bmj.com/content/44/5/598.full
SO  - Gut1999 May 01; 44
AB  - BACKGROUND Bile acid toxicity has been shown in the gastric, colonic, and hepatic tissues; the effect on oesophageal mucosa is less well known.AIMS To determine the spectrum of bile acids refluxing in patients with gastro-oesophageal reflux disease and its relation to oesophageal pH using a new technique of combined oesophageal aspiration and pH monitoring.METHODS Ten asymptomatic subjects and 30 patients with symptoms of gastro-oesophageal reflux disease (minimal mucosal injury, erosive oesophagitis (grade 2 or 3 Savary-Miller), Barrett’s oesophagus/stricture; n=10 in each group) underwent 15 hour continuous oesophageal aspiration with simultaneous pH monitoring. Bile acid assay of the oesophageal samples was performed using modified high performance liquid chromatography.RESULTS The peak bile acid concentration and DeMeester acid scores were significantly higher in the patients with oesophagitis (median bile acid concentration 124 μmol/l; acid score 20.2) and Barrett’s oesophagus/stricture (181 μmol/l; 43.3) than patients with minimal injury (14 μmol/l; 12.5) or controls (0 μmol/l; 11.1). The predominant bile acids detected were cholic, taurocholic, and glycocholic acids but there was a significantly greater proportion of secondary bile acids, deoxycholic and taurodeoxycholic acids, in patients with erosive oesophagitis and Barrett’s oesophagus/stricture. Although bile acid reflux episodes occurred at variable pH, a temporal relation existed between reflux of taurine conjugates and oesophageal acid exposure (r=0.58, p=0.009).CONCLUSION Toxic secondary bile acid fractions have been detected in patients with extensive mucosal damage. Mixed reflux is more harmful than acid reflux alone with possible toxic synergism existing between the taurine conjugates and acid.HPLChigh performance liquid chromatography