RT Journal Article
SR Electronic
T1 Expression and activity of mitogen activated protein kinases in human colorectal carcinoma
JF Gut
JO Gut
FD BMJ Publishing Group Ltd and British Society of Gastroenterology
SP 834
OP 838
DO 10.1136/gut.44.6.834
VO 44
IS 6
A1 S Eggstein
A1 M Franke
A1 I Kutschka
A1 G Manthey
A1 B U von Specht
A1 G Ruf
A1 E H Farthmann
YR 1999
UL http://gut.bmj.com/content/44/6/834.abstract
AB BACKGROUND Mitogen activated protein kinases (MAPKs) play a central role in the regulation of both cell growth and differentiation. They are involved in signal transduction of oncogenes and growth factors. The role of MAPK in colonic carcinoma is unknown.AIMS To establish whether the expression and activity of p42/44 MAPKs are altered in colorectal tumours as compared with normal mucosa.METHODS The expression and activity of p42/p44 MAPK were investigated in 22 colorectal carcinomas, four adenomas, and the corresponding normal colorectal mucosa by the use of western blotting, immunoprecipitation, and in vitro kinase assays.RESULTS After immunoprecipitation with an antibody specific for p42 MAPK, we found significant inactivation of p42 MAPK in colonic carcinomas as well as in adenomas, whereas most sample pairs showed only minor differences in p42 MAPK expression. Investigation of MAPK with an antibody capable of detecting both p42 and p44 MAPK showed a slight but significant decrease in p44 MAPK content in malignant tissues. With this antibody, only minor alterations in MAPK activity and no correlation with p42 MAPK activity were found.CONCLUSIONS Inactivation of p42 MAPK could be associated with colonic carcinogenesis.MAPKmitogen activated protein kinaseSSUseastar unitsSDS/PAGEsodium dodecyl sulphate/polyacrylamide gel electrophoresisMEKMAPK kinase