TY - JOUR T1 - Association of peptic ulcer with increased expression of Lewis antigens but not <em>cagA, iceA, </em>and<em>vacA</em> in <em>Helicobacter pylori</em> isolates in an Asian population JF - Gut JO - Gut SP - 18 LP - 22 DO - 10.1136/gut.47.1.18 VL - 47 IS - 1 AU - P Y Zheng AU - J Hua AU - K G Yeoh AU - B Ho Y1 - 2000/07/01 UR - http://gut.bmj.com/content/47/1/18.abstract N2 - BACKGROUND Studies in Western populations suggest that cagA, iceA, and vacAgene status in Helicobacter pylori isolates is associated with increased virulence and peptic ulcer disease.AIM To investigate the relationship between peptic ulcer and expression of Lewis (Le) antigens as well as cagA, iceA, and vacA inH pylori isolates in Singapore.METHODS Expression of Le antigens in H pylori isolates obtained from patients with dyspepsia was measured by enzyme linked immunosorbent assay. The cagA, iceA, and vacAstatus was determined by polymerase chain reaction.RESULTS Of 108H pylori isolates, 103 (95.4%) expressed Lex and/or Ley, while Lea and Leb were expressed in 23 (21.3%) and 47 (43.5%) isolates, respectively. Expression of two or more Le antigens (Lex, Ley, Lea, or Leb) was significantly higher in H pylori isolated from ulcer patients than in non-ulcer patients (89.6%v 73.2%, p=0.035). There were no significant differences in the prevalence ofcagA or iceA1 inH pylori isolates from peptic ulcer and non-ulcer patients (86.6% v 90.2% forcagA; 70.1% v68.3% for iceA1), and no association of peptic ulcer with any specific vacAgenotype.CONCLUSIONS The present study indicates that peptic ulcer disease is associated with increased expression of Lewis antigens but notcagA, iceA, orvacA genotype in H pylori isolates in our population. This suggests thatcagA, iceA, andvacA are not universal virulence markers, and that host-pathogen interactions are important in determining clinical outcome.cagAcytotoxin associated geneiceAinduced by contact with epithelium genevacAvacuolating cytotoxin geneLeLewis blood group antigenPCRpolymerase chain reactionPUpeptic ulcerLPSlipopolysaccharidesNUDnon-ulcer dyspepsiaODoptical density ER -