PT  - JOURNAL ARTICLE
AU  - S Sakamaki
AU  - N Takayanagi
AU  - N Yoshizaki
AU  - S Hayashi
AU  - T Takayama
AU  - J Kato
AU  - K Kogawa
AU  - N Yamauchi
AU  - N Takemoto
AU  - A Nobuoka
AU  - T Ayabe
AU  - Y Kohgo
AU  - Y Niitsu
TI  - Autoantibodies against the specific epitope of human tropomyosin(s) detected by a peptide based enzyme immunoassay in sera of patients with ulcerative colitis show antibody dependent cell mediated cytotoxicity against HLA-DPw9 transfected L cells
AID  - 10.1136/gut.47.2.236
DP  - 2000 Aug 01
TA  - Gut
PG  - 236--241
VI  - 47
IP  - 2
4099  - http://gut.bmj.com/content/47/2/236.short
4100  - http://gut.bmj.com/content/47/2/236.full
SO  - Gut2000 Aug 01; 47
AB  - BACKGROUND AND AIMS Recent studies suggest that tropomyosin (TM) may act as a putative autoantigen in ulcerative colitis (UC). Recently, we identified, by computer homology analysis, a specific peptide (HIAEDADRK) in human TM that can bind to HLA-DPw9. The aim of this study was to investigate the presence of autoantibodies against this peptide in UC.METHODS Antibodies were measured by ELISA with a synthetic peptide in 20 healthy volunteers, 48 patients with UC, 26 with Crohn's disease (CD), eight with primary sclerosing cholangitis (PSC), and six with primary biliary cirrhosis (PBC). The functional significance of antibodies was investigated by antibody dependent cell mediated cytotoxicity (ADCC) against DPw9 transfected L cells using a standard 51Cr release assay.RESULTS Optical density values (mean (SD)) of sera from patients with UC (1.40 (0.52)) and PSC (1.65 (0.12)) were significantly higher than those from healthy volunteers (0.32 (0.28)) (p<0.05), CD (0.50 (0.34)) (p<0.05) and PBC (0.14 (0.09)) (p<0.05). Values in UC decreased with clinical improvement. The ADCC activity of UC sera correlated well with antibody titre against this synthetic peptide.CONCLUSIONS Anti-TM antibody was detected in UC sera by a specific peptide based ELISA with high reproducibility. This peptide may be an antigenic epitope of TM involved in the immunopathogenesis of UC and, perhaps, PSC.TMtropomyosinUCulcerative colitisCDCrohn's diseasePSCprimary sclerosing cholangitisPBCprimary biliary cirrhosisADCCantibody dependent cell mediated cytotoxicityAAamino acidPBSphosphate buffered salineALPalkaline phosphatasePBMNCperipheral blood mononuclear cellsODoptical density