PT  - JOURNAL ARTICLE
AU  - S M Cruickshank
AU  - L D McVay
AU  - D C Baumgart
AU  - P J Felsburg
AU  - S R Carding
TI  - Colonic epithelial cell mediated suppression of CD4 T cell activation
AID  - 10.1136/gut.2003.029967
DP  - 2004 May 01
TA  - Gut
PG  - 678--684
VI  - 53
IP  - 5
4099  - http://gut.bmj.com/content/53/5/678.short
4100  - http://gut.bmj.com/content/53/5/678.full
SO  - Gut2004 May 01; 53
AB  - Background and aims: As the first point of contact with enteric antigens, intestinal epithelial cells (IEC) may be key in regulating mucosal immune responses. We determined therefore if murine colonic epithelial cells (CEC) have tolerogenic or activating effects on CD4 T cells. Methods: Using a novel CEC, macrophages, and CD4 T cell coculture system, mitogen and antigen specific responses of naïve and antigen primed CD4 T cells were assessed. Results: Although a proportion of CEC express the costimulatory molecules B7.1, B7.2, CD40, and CD54, they were unable to promote mitogen or antigen driven activation of CD4 T cells, even in the presence of exogenous costimulatory signals. CD4 T cells cocultured with CEC were CD25lo and CD45RBlo and remained in the G1 phase of the cell cycle. CEC were also able to prevent CD4 T cell activation by professional antigen presenting cells. CEC mediated suppression of T cell activation was cell contact dependent and transforming growth factor β independent. Conclusions: These observations suggest that CEC contribute to the maintenance of T cell tolerance in the gut by preventing inappropriate activation of CD4 T cells.