PT - JOURNAL ARTICLE AU - I Castillo AU - E Rodríguez-Iñigo AU - J Bartolomé AU - S de Lucas AU - N Ortíz-Movilla AU - J M López-Alcorocho AU - M Pardo AU - V Carreño TI - Hepatitis C virus replicates in peripheral blood mononuclear cells of patients with occult hepatitis C virus infection AID - 10.1136/gut.2004.057281 DP - 2005 May 01 TA - Gut PG - 682--685 VI - 54 IP - 5 4099 - http://gut.bmj.com/content/54/5/682.short 4100 - http://gut.bmj.com/content/54/5/682.full SO - Gut2005 May 01; 54 AB - Background: Occult hepatitis C virus (HCV) infection is characterised by the presence of HCV-RNA in the liver in the absence of anti-HCV, and serum viral RNA. Up to 70% of these patients also have HCV-RNA in peripheral blood mononuclear cells (PBMC) but it is not known if HCV is replicating in these cells. Aim: We studied possible HCV replication in PBMC of 18 patients with an occult HCV infection who were selected on the basis of HCV-RNA positivity in PBMC. Methods: Detection of HCV-RNA positive and negative strands in PBMC was done by strand specific reverse transcriptase-polymerase chain reaction (RT-PCR) and by in situ hybridisation. Results: The presence of HCV-RNA positive strand in PBMC was confirmed in all patients by strand specific RT-PCR and by in situ hybridisation. Mean percentage of PBMC which had the HCV-RNA positive strand was 3.3% (95% confidence interval (CI) 2.1–4.4) The HCV-RNA negative strand was found in the PBMC of 11/18 (61%) patients by strand specific RT-PCR and confirmed by in situ hybridisation, and the percentage of PBMC harbouring the HCV-RNA negative strand was 3.1% (95% CI 0.8–5.5). There was a significant correlation (p = 0.001, r = 0.84) between the percentage of PBMC with the HCV-RNA positive strand and that of PBMC with the HCV-RNA negative strand. Conclusion: HCV replicates in the PBMC of patients with occult HCV infection and thus, although these patients do not have serum HCV-RNA, they could be potentially infectious.