RT Journal Article
SR Electronic
T1 Dietary folate, alcohol and B vitamins in relation to LINE-1 hypomethylation in colon cancer
JF Gut
JO Gut
FD BMJ Publishing Group Ltd and British Society of Gastroenterology
SP 794
OP 799
DO 10.1136/gut.2009.183707
VO 59
IS 6
A1 Eva S Schernhammer
A1 Edward Giovannucci
A1 Takako Kawasaki
A1 Bernard Rosner
A1 Charles S Fuchs
A1 Shuji Ogino
YR 2010
UL http://gut.bmj.com/content/59/6/794.abstract
AB Background and aims Although critical for methylation reactions, how dietary folate and B vitamins affect global DNA methylation level in colorectal cancers is currently unknown. Long interspersed nucleotide element-1 (LINE-1) is an emerging indicator of genome-wide DNA methylation level that has previously been linked to colon cancer survival.Methods We examined the association between dietary intake of folate, alcohol and B vitamins and LINE-1 hypomethylation in 609 incident colon cancers, utilising the database of two independent prospective cohort studies.Results Participants with ≥400 μg folate intake per day were significantly less likely to develop LINE-1 hypomethylated colon cancers than those reporting <200 μg of folate intake per day (RR=0.57, 95% CI=0.36 to 0.91 for <55% LINE-1 methylated colon tumours; RR=0.74, 95% CI=0.51 to 1.06 for 55–64% LINE-1 methylated colon tumours; and RR=1.08, 95% CI=0.66 to 1.75 for ≥65% LINE-1 methylated tumours; Pinteraction=0.01). By contrast, high alcohol consumption conferred a higher risk of LINE-1 hypomethylated cancers (≥15 g alcohol per day versus none, RR=1.67, 95% CI=1.04 to 2.67 for <55% LINE1 methylated tumours; and RR=1.55, 95% CI=1.10 to 2.18 for 55–64% LINE-1 methylated tumours) but had no association with ≥65% LINE-1 methylated tumours (RR=1.06, 95% CI=0.69 to 1.62). High intakes of vitamin B6, B12 or methionine were not significantly associated with colon cancers, regardless of LINE-1 methylation level.Conclusion The influence of dietary folate intake and alcohol consumption on colon cancer risk differs significantly according to tumoral LINE-1 methylation level.