%0 Journal Article %A Erwin Daniel Brenndörfer %A Anette Brass %A Jonas Söderholm %A Lars Frelin %A Soo Aleman %A Johannes Georg Bode %A Matti Sällberg %T Hepatitis C virus non-structural 3/4A protein interferes with intrahepatic interferon-γ production %D 2012 %R 10.1136/gut.2010.232116 %J Gut %P 589-596 %V 61 %N 4 %X Background The non-structural (NS) 3/4A protease/helicase of the hepatitis C virus is known to modulate signalling pathways in the infected hepatocyte by cleaving CARD adaptor inducing IFNβ (Cardif), T-cell protein tyrosine phosphatase (TC-PTP) and TIR domain-containing adaptor inducing IFNβ (TRIF), but the effects of NS3/4A in vivo still remain unclear.Aim To investigate the influence of NS3/4A on intracellular and intercellular signalling in vivo by analysing the intrahepatic inflammatory response of naïve, lipopolysaccharide (LPS)/d-galactosamine (d-galN) or tumour necrosis factor-α (TNFα)/d-galN-treated NS3/4A-transgenic (Tg) mice.Methods The intrahepatic immunity of naïve and LPS/d-galN- or TNFα/d-galN-treated NS3/4A-Tg mice was determined using western blot, ELISA, real-time PCR, flow cytometry and survival monitoring. The injection of cytokines or antibodies against signalling components was performed to analyse the relevance of the respective pathways for the investigated issues. A Tg mouse lineage expressing an inactivated NS3/4A protease (NS3/4AIle1073Ala-Tgs) was generated to examine if protective effects were NS3/4A protease dependent.Results The activation of hepatic signal transducer and activator of transcription 1 and 2 was impaired in NS3/4A-Tg mice after treatment with LPS/d-galN or TNFα/d-galN. This was paralleled by a reduction in hepatic interferon-γ (IFNγ). Reconstitution of IFNγ reverted the resistance to LPS/TNFα in NS3/4A-Tg mice. Subsequently, blocking IFNγ in vivo rendered wild-type mice resistant against treatment with LPS/TNFα. A new Tg mouse expressing an inactivated NS3/4A protease had the same phenotype as wild-type mice with respect to hepatic IFNγ levels and sensitivity to LPS/d-galN. Finally, the chemokine profile was altered in the NS3/4A-Tg mice towards an anti-inflammatory state, which helps to explain the altered immune cell subsets and reduction in hepatic IFNγ production.Conclusions Our data demonstrate that the NS3/4A protease reduces the intrahepatic production of IFNγ and alters TNFα-mediated effects, thereby impairing the hepatic inflammatory response. This may contribute to viral persistence. %U https://gut.bmj.com/content/gutjnl/61/4/589.full.pdf