PT - JOURNAL ARTICLE AU - Josef Ehling AU - Matthias Bartneck AU - Xiao Wei AU - Felix Gremse AU - Viktor Fech AU - Diana Möckel AU - Christer Baeck AU - Kanishka Hittatiya AU - Dirk Eulberg AU - Tom Luedde AU - Fabian Kiessling AU - Christian Trautwein AU - Twan Lammers AU - Frank Tacke TI - CCL2-dependent infiltrating macrophages promote angiogenesis in progressive liver fibrosis AID - 10.1136/gutjnl-2013-306294 DP - 2014 Dec 01 TA - Gut PG - 1960--1971 VI - 63 IP - 12 4099 - http://gut.bmj.com/content/63/12/1960.short 4100 - http://gut.bmj.com/content/63/12/1960.full SO - Gut2014 Dec 01; 63 AB - Objectives In chronic liver injury, angiogenesis, the formation of new blood vessels from pre-existing ones, may contribute to progressive hepatic fibrosis and to development of hepatocellular carcinoma. Although hypoxia-induced expression of vascular endothelial growth factor (VEGF) occurs in advanced fibrosis, we hypothesised that inflammation may endorse hepatic angiogenesis already at early stages of fibrosis. Design Angiogenesis in livers of c57BL/6 mice upon carbon tetrachloride- or bile duct ligation-induced chronic hepatic injury was non-invasively monitored using in vivo contrast-enhanced micro computed tomography (µCT) and ex vivo anatomical µCT after hepatic Microfil perfusion. Functional contributions of monocyte-derived macrophage subsets for angiogenesis were explored by pharmacological inhibition of CCL2 using the Spiegelmer mNOX-E36. Results Contrast-enhanced in vivo µCT imaging allowed non-invasive monitoring of the close correlation of angiogenesis, reflected by functional hepatic blood vessel expansion, with experimental fibrosis progression. On a cellular level, inflammatory monocyte-derived macrophages massively accumulated in injured livers, colocalised with newly formed vessels in portal tracts and exhibited pro-angiogenic gene profiles including upregulated VEGF and MMP9. Functional in vivo and anatomical ex vivo µCT analyses demonstrated that inhibition of monocyte infiltration by targeting the chemokine CCL2 prevented fibrosis-associated angiogenesis, but not fibrosis progression. Monocyte-derived macrophages primarily fostered sprouting angiogenesis within the portal vein tract. Portal vein diameter as a measure of portal hypertension depended on fibrosis, but not on angiogenesis. Conclusions Inflammation-associated angiogenesis is promoted by CCL2-dependent monocytes during fibrosis progression. Innovative in vivo µCT methodology can accurately monitor angiogenesis and antiangiogenic therapy effects in experimental liver fibrosis.