PT  - JOURNAL ARTICLE
AU  - M Olivares
AU  - A Neef
AU  - G Castillejo
AU  - G De Palma
AU  - V Varea
AU  - A Capilla
AU  - F Palau
AU  - E Nova
AU  - A Marcos
AU  - I Polanco
AU  - C Ribes-Koninckx
AU  - L Ortigosa
AU  - L Izquierdo
AU  - Y Sanz
TI  - The HLA-DQ2 genotype selects for early intestinal microbiota composition in infants at high risk of developing coeliac disease
AID  - 10.1136/gutjnl-2014-306931
DP  - 2015 Mar 01
TA  - Gut
PG  - 406--417
VI  - 64
IP  - 3
4099  - http://gut.bmj.com/content/64/3/406.short
4100  - http://gut.bmj.com/content/64/3/406.full
SO  - Gut2015 Mar 01; 64
AB  - Objective Intestinal dysbiosis has been associated with coeliac disease (CD), but whether the alterations are cause or consequence of the disease is unknown. This study investigated whether the human leukocyte antigen (HLA)-DQ2 genotype is an independent factor influencing the early gut microbiota composition of healthy infants at family risk of CD. Design As part of a larger prospective study, a subset (n=22) of exclusively breastfed and vaginally delivered infants with either high genetic risk (HLA-DQ2 carriers) or low genetic risk (non-HLA-DQ2/8 carriers) of developing CD were selected from a cohort of healthy infants with at least one first-degree relative with CD. Infant faecal microbiota was analysed by 16S rRNA gene pyrosequencing and real time quantitative PCR. Results Infants with a high genetic risk had significantly higher proportions of Firmicutes and Proteobacteria and lower proportions of Actinobacteria compared with low-risk infants. At genus level, high-risk infants had significantly less Bifidobacterium and unclassified Bifidobacteriaceae proportions and more Corynebacterium, Gemella, Clostridium sensu stricto, unclassified Clostridiaceae, unclassified Enterobacteriaceae and Raoultella proportions. Quantitative real time PCR also revealed lower numbers of Bifidobacterium species in infants with high genetic risk than in those with low genetic risk. In high-risk infants negative correlations were identified between Bifidobacterium species and several genera of Proteobacteria (Escherichia/Shigella) and Firmicutes (Clostridium). Conclusions The genotype of infants at family risk of developing CD, carrying the HLA-DQ2 haplotypes, influences the early gut microbiota composition. This finding suggests that a specific disease-biased host genotype may also select for the first gut colonisers and could contribute to determining disease risk.