PT  - JOURNAL ARTICLE
AU  - Martin Kummen
AU  - Kristian Holm
AU  - Jarl Andreas Anmarkrud
AU  - Ståle Nygård
AU  - Mette Vesterhus
AU  - Marte L Høivik
AU  - Marius Trøseid
AU  - Hanns-Ulrich Marschall
AU  - Erik Schrumpf
AU  - Bjørn Moum
AU  - Helge Røsjø
AU  - Pål Aukrust
AU  - Tom H Karlsen
AU  - Johannes R Hov
TI  - The gut microbial profile in patients with primary sclerosing cholangitis is distinct from patients with ulcerative colitis without biliary disease and healthy controls
AID  - 10.1136/gutjnl-2015-310500
DP  - 2017 Apr 01
TA  - Gut
PG  - 611--619
VI  - 66
IP  - 4
4099  - http://gut.bmj.com/content/66/4/611.short
4100  - http://gut.bmj.com/content/66/4/611.full
SO  - Gut2017 Apr 01; 66
AB  - Objective Gut microbiota could influence gut, as well as hepatic and biliary immune responses. We therefore thoroughly characterised the gut microbiota in primary sclerosing cholangitis (PSC) compared with healthy controls (HC) and patients with ulcerative colitis without liver disease.Design We prospectively collected 543 stool samples. After a stringent exclusion process, bacterial DNA was submitted for 16S rRNA gene sequencing. PSC and HC were randomised to an exploration panel or a validation panel, and only significant results (p<0.05, QFDR<0.20) in both panels were reported, followed by a combined comparison of all samples against UC.Results Patients with PSC (N=85) had markedly reduced bacterial diversity compared with HC (N=263, p<0.0001), and a different global microbial composition compared with both HC (p<0.001) and UC (N=36, p<0.01). The microbiota of patients with PSC with and without IBD was similar. Twelve genera separated PSC and HC, out of which 11 were reduced in PSC. However, the Veillonella genus showed a marked increase in PSC compared with both HC (p<0.0001) and UC (p<0.02). Using receiver operating characteristic analysis, Veillonella abundance yielded an area under the curve (AUC) of 0.64 to discriminate PSC from HC, while a combination of PSC-associated genera yielded an AUC of 0.78.Conclusions Patients with PSC exhibited a gut microbial signature distinct from both HC and UC without liver disease, but similar in PSC with and without IBD. The Veillonella genus, which is also associated with other chronic inflammatory and fibrotic conditions, was enriched in PSC.