@article {Yazici1983, author = {Cemal Yazici and Patricia G Wolf and Hajwa Kim and Tzu-Wen L Cross and Karin Vermillion and Timothy Carroll and Gaius J Augustus and Ece Mutlu and Lisa Tussing-Humphreys and Carol Braunschweig and Rosa M Xicola and Barbara Jung and Xavier Llor and Nathan A Ellis and H Rex Gaskins}, title = {Race-dependent association of sulfidogenic bacteria with colorectal cancer}, volume = {66}, number = {11}, pages = {1983--1994}, year = {2017}, doi = {10.1136/gutjnl-2016-313321}, publisher = {BMJ Publishing Group}, abstract = {Objective Colorectal cancer (CRC) incidence is higher in African Americans (AAs) compared with non-Hispanic whites (NHWs). A diet high in animal protein and fat is an environmental risk factor for CRC development. The intestinal microbiota is postulated to modulate the effects of diet in promoting or preventing CRC. Hydrogen sulfide, produced by autochthonous sulfidogenic bacteria, triggers proinflammatory pathways and hyperproliferation, and is genotoxic. We hypothesised that sulfidogenic bacterial abundance in colonic mucosa may be an environmental CRC risk factor that distinguishes AA and NHW.Design Colonic biopsies from uninvolved or healthy mucosa from CRC cases and tumour-free controls were collected prospectively from five medical centres in Chicago for association studies. Sulfidogenic bacterial abundance in uninvolved colonic mucosa of AA and NHW CRC cases was compared with normal mucosa of AA and NHW controls. In addition, 16S rDNA sequencing was performed in AA cases and controls. Correlations were examined among bacterial targets, race, disease status and dietary intake.Results AAs harboured a greater abundance of sulfidogenic bacteria compared with NHWs regardless of disease status. Bilophila wadsworthia-specific dsrA was more abundant in AA cases than controls. Linear discriminant analysis of 16S rRNA gene sequences revealed five sulfidogenic genera that were more abundant in AA cases. Fat and protein intake and daily servings of meat were significantly higher in AAs compared with NHWs, and multiple dietary components correlated with a higher abundance of sulfidogenic bacteria.Conclusions These results implicate sulfidogenic bacteria as a potential environmental risk factor contributing to CRC development in AAs.}, issn = {0017-5749}, URL = {https://gut.bmj.com/content/66/11/1983}, eprint = {https://gut.bmj.com/content/66/11/1983.full.pdf}, journal = {Gut} }