TY - JOUR T1 - Dysbiosis of maternal and neonatal microbiota associated with gestational diabetes mellitus JF - Gut JO - Gut DO - 10.1136/gutjnl-2018-315988 SP - gutjnl-2018-315988 AU - Jinfeng Wang AU - Jiayong Zheng AU - Wenyu Shi AU - Nan Du AU - Xiaomin Xu AU - Yanming Zhang AU - Peifeng Ji AU - Fengyi Zhang AU - Zhen Jia AU - Yeping Wang AU - Zhi Zheng AU - Hongping Zhang AU - Fangqing Zhao Y1 - 2018/05/14 UR - http://gut.bmj.com/content/early/2018/05/14/gutjnl-2018-315988.abstract N2 - Objective The initial colonisation of the human microbiota and the impact of maternal health on neonatal microbiota at birth remain largely unknown. The aim of our study is to investigate the possible dysbiosis of maternal and neonatal microbiota associated with gestational diabetes mellitus (GDM) and to estimate the potential risks of the microbial shift to neonates.Design Pregnant women and neonates suffering from GDM were enrolled and 581 maternal (oral, intestinal and vaginal) and 248 neonatal (oral, pharyngeal, meconium and amniotic fluid) samples were collected. To avoid vaginal bacteria contaminations, the included neonates were predominantly delivered by C-section, with their samples collected within seconds of delivery.Results Numerous and diverse bacterial taxa were identified from the neonatal samples, and the samples from different neonatal body sites were grouped into distinct clusters. The microbiota of pregnant women and neonates was remarkably altered in GDM, with a strong correlation between certain discriminatory bacteria and the oral glucose tolerance test. Microbes varying by the same trend across the maternal and neonatal microbiota were observed, revealing the intergenerational concordance of microbial variation associated with GDM. Furthermore, lower evenness but more depletion of KEGG orthologues and higher abundance of some viruses (eg, herpesvirus and mastadenovirus) were observed in the meconium microbiota of neonates associated with GDM.Conclusion GDM can alter the microbiota of both pregnant women and neonates at birth, which sheds light on another form of inheritance and highlights the importance of understanding the formation of early-life microbiome. ER -