TY - JOUR T1 - <em>Akkermansia muciniphila</em> induces gut microbiota remodelling and controls islet autoimmunity in NOD mice JF - Gut JO - Gut SP - 1445 LP - 1453 DO - 10.1136/gutjnl-2017-314508 VL - 67 IS - 8 AU - Arno Hänninen AU - Raine Toivonen AU - Sakari Pöysti AU - Clara Belzer AU - Hubert Plovier AU - Janneke P Ouwerkerk AU - Rohini Emani AU - Patrice D Cani AU - Willem M De Vos Y1 - 2018/08/01 UR - http://gut.bmj.com/content/67/8/1445.abstract N2 - Objective Intestinal microbiota is implicated in the pathogenesis of autoimmune type 1 diabetes in humans and in non-obese diabetic (NOD) mice, but evidence on its causality and on the role of individual microbiota members is limited. We investigated if different diabetes incidence in two NOD colonies was due to microbiota differences and aimed to identify individual microbiota members with potential significance.Design We profiled intestinal microbiota between two NOD mouse colonies showing high or low diabetes incidence by 16S ribosomal RNA gene sequencing and colonised the high-incidence colony with the microbiota of the low-incidence colony. Based on unaltered incidence, we identified a few taxa which were not effectively transferred and thereafter, transferred experimentally one of these to test its potential significance.Results Although the high-incidence colony adopted most microbial taxa present in the low-incidence colony, diabetes incidence remained unaltered. Among the few taxa which were not transferred, Akkermansia muciniphila was identified. As A. muciniphila abundancy is inversely correlated to the risk of developing type 1 diabetes-related autoantibodies, we transferred A. muciniphila experimentally to the high-incidence colony. A. muciniphila transfer promoted mucus production and increased expression of antimicrobial peptide Reg3γ, outcompeted Ruminococcus torques from the microbiota, lowered serum endotoxin levels and islet toll-like receptor expression, promoted regulatory immunity and delayed diabetes development.Conclusion Transfer of the whole microbiota may not reduce diabetes incidence despite a major change in gut microbiota, but single symbionts such as A. muciniphila with beneficial metabolic and immune signalling effects may reduce diabetes incidence when administered as a probiotic. ER -