PT  - JOURNAL ARTICLE
AU  - Jinfeng Wang
AU  - Jiayong Zheng
AU  - Wenyu Shi
AU  - Nan Du
AU  - Xiaomin Xu
AU  - Yanming Zhang
AU  - Peifeng Ji
AU  - Fengyi Zhang
AU  - Zhen Jia
AU  - Yeping Wang
AU  - Zhi Zheng
AU  - Hongping Zhang
AU  - Fangqing Zhao
TI  - Dysbiosis of maternal and neonatal microbiota associated with gestational diabetes mellitus
AID  - 10.1136/gutjnl-2018-315988
DP  - 2018 Sep 01
TA  - Gut
PG  - 1614--1625
VI  - 67
IP  - 9
4099  - http://gut.bmj.com/content/67/9/1614.short
4100  - http://gut.bmj.com/content/67/9/1614.full
SO  - Gut2018 Sep 01; 67
AB  - Objective The initial colonisation of the human microbiota and the impact of maternal health on neonatal microbiota at birth remain largely unknown. The aim of our study is to investigate the possible dysbiosis of maternal and neonatal microbiota associated with gestational diabetes mellitus (GDM) and to estimate the potential risks of the microbial shift to neonates.Design Pregnant women and neonates suffering from GDM were enrolled and 581 maternal (oral, intestinal and vaginal) and 248 neonatal (oral, pharyngeal, meconium and amniotic fluid) samples were collected. To avoid vaginal bacteria contaminations, the included neonates were predominantly delivered by C-section, with their samples collected within seconds of delivery.Results Numerous and diverse bacterial taxa were identified from the neonatal samples, and the samples from different neonatal body sites were grouped into distinct clusters. The microbiota of pregnant women and neonates was remarkably altered in GDM, with a strong correlation between certain discriminatory bacteria and the oral glucose tolerance test. Microbes varying by the same trend across the maternal and neonatal microbiota were observed, revealing the intergenerational concordance of microbial variation associated with GDM. Furthermore, lower evenness but more depletion of KEGG orthologues and higher abundance of some viruses (eg, herpesvirus and mastadenovirus) were observed in the meconium microbiota of neonates associated with GDM.Conclusion GDM can alter the microbiota of both pregnant women and neonates at birth, which sheds light on another form of inheritance and highlights the importance of understanding the formation of early-life microbiome.