TY - JOUR T1 - Donor metabolic characteristics drive effects of faecal microbiota transplantation on recipient insulin sensitivity, energy expenditure and intestinal transit time JF - Gut JO - Gut SP - 502 LP - 512 DO - 10.1136/gutjnl-2019-318320 VL - 69 IS - 3 AU - Pieter de Groot AU - Torsten Scheithauer AU - Guido J Bakker AU - Andrei Prodan AU - Evgeni Levin AU - Muhammad Tanweer Khan AU - Hilde Herrema AU - Mariette Ackermans AU - Mireille J M Serlie AU - Maurits de Brauw AU - Johannes H M Levels AU - Amber Sales AU - Victor E Gerdes AU - Marcus Ståhlman AU - Alinda W M Schimmel AU - Geesje Dallinga-Thie AU - Jacques JGHM Bergman AU - Frits Holleman AU - Joost B L Hoekstra AU - Albert Groen AU - Fredrik Bäckhed AU - Max Nieuwdorp Y1 - 2020/03/01 UR - http://gut.bmj.com/content/69/3/502.abstract N2 - Objective Bariatric surgery improves glucose metabolism. Recent data suggest that faecal microbiota transplantation (FMT) using faeces from postbariatric surgery diet-induced obese mice in germ-free mice improves glucose metabolism and intestinal homeostasis. We here investigated whether allogenic FMT using faeces from post-Roux-en-Y gastric bypass donors (RYGB-D) compared with using faeces from metabolic syndrome donors (METS-D) has short-term effects on glucose metabolism, intestinal transit time and adipose tissue inflammation in treatment-naïve, obese, insulin-resistant male subjects.Design Subjects with metabolic syndrome (n=22) received allogenic FMT either from RYGB-D or METS-D. Hepatic and peripheral insulin sensitivity as well as lipolysis were measured at baseline and 2 weeks after FMT by hyperinsulinaemic euglycaemic stable isotope (2H2-glucose and 2H5-glycerol) clamp. Secondary outcome parameters were changes in resting energy expenditure, intestinal transit time, faecal short-chain fatty acids (SCFA) and bile acids, and inflammatory markers in subcutaneous adipose tissue related to intestinal microbiota composition. Faecal SCFA, bile acids, glycaemic control and inflammatory parameters were also evaluated at 8 weeks.Results We observed a significant decrease in insulin sensitivity 2 weeks after allogenic METS-D FMT (median rate of glucose disappearance: from 40.6 to 34.0 µmol/kg/min; p<0.01). Moreover, a trend (p=0.052) towards faster intestinal transit time following RYGB-D FMT was seen. Finally, we observed changes in faecal bile acids (increased lithocholic, deoxycholic and (iso)lithocholic acid after METS-D FMT), inflammatory markers (decreased adipose tissue chemokine ligand 2 (CCL2) gene expression and plasma CCL2 after RYGB-D FMT) and changes in several intestinal microbiota taxa.Conclusion Allogenic FMT using METS-D decreases insulin sensitivity in metabolic syndrome recipients when compared with using post-RYGB-D. Further research is needed to delineate the role of donor characteristics in FMT efficacy in human insulin-resistant subjects.Trial registration number NTR4327. ER -