PT  - JOURNAL ARTICLE
AU  - Jianbo Tian
AU  - Ying Zhu
AU  - Meilin Rao
AU  - Yimin Cai
AU  - Zequn Lu
AU  - Danyi Zou
AU  - Xiating Peng
AU  - Pingting Ying
AU  - Ming Zhang
AU  - Siyuan Niu
AU  - Yue Li
AU  - Rong Zhong
AU  - Jiang Chang
AU  - Xiaoping Miao
TI  - N<sup>6</sup>-methyladenosine mRNA methylation of <em>PIK3CB</em> regulates AKT signalling to promote PTEN-deficient pancreatic cancer progression
AID  - 10.1136/gutjnl-2019-320179
DP  - 2020 Apr 19
TA  - Gut
PG  - gutjnl-2019-320179
4099  - http://gut.bmj.com/content/early/2020/04/19/gutjnl-2019-320179.short
4100  - http://gut.bmj.com/content/early/2020/04/19/gutjnl-2019-320179.full
AB  - Objective Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal cancers worldwide. Thus far, most drugs have failed to significantly improve patient survival. N6-methyladenosine (m6A) plays an important role in the progression of PDAC, but its aberrant regulation driven by germline variants in human diseases remains unclear.Design We first performed an exome-wide association analysis in 518 PDAC patients with overall survival and replicated in an independent population containing 552 PDAC patients. Then, a series of biochemical experiments in vitro and in vivo were conducted to investigate potential mechanisms of the candidate variant and its target gene PIK3CB underlying the PDAC progression. Moreover, the PIK3CB-selective inhibitor KIN-193 was used to block PDAC tumour growth.Results We identified a missense variant rs142933486 in PIK3CB that is significantly associated with the overall survival of PDAC by reducing the PIK3CB m6A level, which facilitated its mRNA and protein expression levels mediated by the m6A ‘writer’ complex (METTL13/METTL14/WTAP) and the m6A ‘reader’ YTHDF2. The upregulation of PIK3CB is widely found in PDAC tumour tissues and significantly correlated with the poor prognosis of PDAC, especially in PTEN-deficient patients. We further demonstrated that PIK3CB overexpression substantially enhanced the proliferation and migration abilities of PTEN-deficient PDAC cells and activated AKT signalling pathway. Remarkably, KIN-193, a PIK3CB-selective inhibitor, is shown to serve as an effective anticancer agent for blocking PTEN-deficient PDAC.Conclusions These findings demonstrate aberrant m6A homoeostasis as an oncogenic mechanism in PDAC and highlight the potential of PIK3CB as a therapeutic target for this disease.