RT Journal Article
SR Electronic
T1 Influence of gastrectomy for gastric cancer treatment on faecal microbiome and metabolome profiles
JF Gut
JO Gut
FD BMJ Publishing Group Ltd and British Society of Gastroenterology
SP 1404
OP 1415
DO 10.1136/gutjnl-2019-319188
VO 69
IS 8
A1 Pande Putu Erawijantari
A1 Sayaka Mizutani
A1 Hirotsugu Shiroma
A1 Satoshi Shiba
A1 Takeshi Nakajima
A1 Taku Sakamoto
A1 Yutaka Saito
A1 Shinji Fukuda
A1 Shinichi Yachida
A1 Takuji Yamada
YR 2020
UL http://gut.bmj.com/content/69/8/1404.abstract
AB Objective Recent evidence points to the gut microbiome’s involvement in postoperative outcomes, including after gastrectomy. Here, we investigated the influence of gastrectomy for gastric cancer on the gut microbiome and metabolome, and how it related to postgastrectomy conditions.Design We performed shotgun metagenomics sequencing and capillary electrophoresis time-of-flight mass spectrometry-based metabolomics analyses on faecal samples collected from participants with a history of gastrectomy for gastric cancer (n=50) and compared them with control participants (n=56).Results The gut microbiota in the gastrectomy group showed higher species diversity and richness (p<0.05), together with greater abundance of aerobes, facultative anaerobes and oral microbes. Moreover, bile acids such as genotoxic deoxycholic acid and branched-chain amino acids were differentially abundant between the two groups (linear discriminant analysis (LDA) effect size (LEfSe): p<0.05, q<0.1, LDA>2.0), as were also Kyoto Encyclopedia of Genes and Genomes modules involved in nutrient transport and organic compounds biosynthesis (LEfSe: p<0.05, q<0.1, LDA>2.0).Conclusion Our results reveal alterations of gut microbiota after gastrectomy, suggesting its association with postoperative comorbidities. The multi-omic approach applied in this study could complement the follow-up of patients after gastrectomy.