TY - JOUR T1 - A purified membrane protein from <em>Akkermansia muciniphila</em> or the pasteurised bacterium blunts colitis associated tumourigenesis by modulation of CD8<sup>+</sup> T cells in mice JF - Gut JO - Gut SP - 1988 LP - 1997 DO - 10.1136/gutjnl-2019-320105 VL - 69 IS - 11 AU - Lijuan Wang AU - Lei Tang AU - Yiming Feng AU - Suying Zhao AU - Mei Han AU - Chuan Zhang AU - Gehui Yuan AU - Jun Zhu AU - Shuyuan Cao AU - Qian Wu AU - Lei Li AU - Zhan Zhang Y1 - 2020/11/01 UR - http://gut.bmj.com/content/69/11/1988.abstract N2 - Objective Gut microbiota have been linked to inflammatory bowel disease (IBD) and colorectal cancer (CRC). Akkermansia muciniphila (A. muciniphila) is a gram-negative anaerobic bacterium that is selectively decreased in the faecal microbiota of patients with IBD, but its causative role and molecular mechanism in blunting colitis-associated colorectal cancer (CAC) remain inconclusive. This study investigates how A. muciniphila engages the immune response in CAC.Design Mice were given dextran sulfate sodium to induce colitis, followed by azoxymethane to establish CAC with or without pasteurised A. muciniphila or a specific outer membrane protein (Amuc_1100) treatment. Faeces from mice and patients with IBD or CRC were collected for 16S rRNA sequencing. The effects of A. muciniphila or Amuc_1100 on the immune response in acute colitis and CAC were investigated.Results A. muciniphila was significantly reduced in patients with IBD and mice with colitis or CAC. A. muciniphila or Amuc_1100 could improve colitis, with a reduction in infiltrating macrophages and CD8+ cytotoxic T lymphocytes (CTLs) in the colon. Their treatment also decreased CD16/32+ macrophages in the spleen and mesenteric lymph nodes (MLN) of colitis mice. Amuc_1100 elevated PD-1+ CTLs in the spleen. Moreover, A. muciniphila and Amuc_1100 blunted tumourigenesis by expanding CTLs in the colon and MLN. Remarkably, they activated CTLs in the MLN, as indicated by TNF-α induction and PD-1downregulation. Amuc_1100 could stimulate and activate CTLs from splenocytes in CT26 cell conditioned medium.Conclusions These data indicate that pasteurised A. muciniphila or Amuc_1100 can blunt colitis and CAC through the modulation of CTLs. ER -