TY - JOUR T1 - Alterations in the human oral and gut microbiomes and lipidomics in COVID-19 JF - Gut JO - Gut SP - 1253 LP - 1265 DO - 10.1136/gutjnl-2020-323826 VL - 70 IS - 7 AU - Zhigang Ren AU - Haiyu Wang AU - Guangying Cui AU - Haifeng Lu AU - Ling Wang AU - Hong Luo AU - Xinhua Chen AU - Hongyan Ren AU - Ranran Sun AU - Wenli Liu AU - Xiaorui Liu AU - Chao Liu AU - Ang Li AU - Xuemei Wang AU - Benchen Rao AU - Chengyu Yuan AU - Hua Zhang AU - Jiarui Sun AU - Xiaolong Chen AU - Bingjie Li AU - Chuansong Hu AU - Zhongwen Wu AU - Zujiang Yu AU - Quancheng Kan AU - Lanjuan Li Y1 - 2021/07/01 UR - http://gut.bmj.com/content/70/7/1253.abstract N2 - Objective To characterise the oral microbiome, gut microbiome and serum lipid profiles in patients with active COVID-19 and recovered patients; evaluate the potential of the microbiome as a non-invasive biomarker for COVID-19; and explore correlations between the microbiome and lipid profile.Design We collected and sequenced 392 tongue-coating samples, 172 faecal samples and 155 serum samples from Central China and East China. We characterised microbiome and lipid molecules, constructed microbial classifiers in discovery cohort and verified their diagnostic potential in 74 confirmed patients (CPs) from East China and 37 suspected patients (SPs) with IgG positivity.Results Oral and faecal microbial diversity was significantly decreased in CPs versus healthy controls (HCs). Compared with HCs, butyric acid-producing bacteria were decreased and lipopolysaccharide-producing bacteria were increased in CPs in oral cavity. The classifiers based on 8 optimal oral microbial markers (7 faecal microbial markers) achieved good diagnostic efficiency in different cohorts. Importantly, diagnostic efficacy reached 87.24% in the cross-regional cohort. Moreover, the classifiers successfully diagnosed SPs with IgG antibody positivity as CPs, and diagnostic efficacy reached 92.11% (98.01% of faecal microbiome). Compared with CPs, 47 lipid molecules, including sphingomyelin (SM)(d40:4), SM(d38:5) and monoglyceride(33:5), were depleted, and 122 lipid molecules, including phosphatidylcholine(36:4p), phosphatidylethanolamine (PE)(16:0p/20:5) and diglyceride(20:1/18:2), were enriched in confirmed patients recovery.Conclusion This study is the first to characterise the oral microbiome in COVID-19, and oral microbiomes and lipid alterations in recovered patients, to explore their correlations and to report the successful establishment and validation of a diagnostic model for COVID-19.Data are available in a public, open access repository. The raw Illumina read data for all samples were deposited in the European Bioinformatics Institute European Nucleotide Archive database (PRJNA660302). ER -