TY - JOUR T1 - Effect of rapid colonic transit on stool microbiome and short-chain fatty acids in diarrhoea-predominant irritable bowel syndrome JF - Gut JO - Gut DO - 10.1136/gutjnl-2022-329359 SP - gutjnl-2022-329359 AU - Joelle BouSaba AU - Ting Zheng AU - Saam Dilmaghani AU - Stephen Johnson AU - Jun Chen AU - Michael Camilleri Y1 - 2023/01/19 UR - http://gut.bmj.com/content/early/2023/01/18/gutjnl-2022-329359.abstract N2 - In a recent article in GUT,1 we showed that, among 194 patients with diarrhoea-predominant irritable bowel syndrome (IBS-D), 43 had altered bile acid (BA) metabolism (ABAM) (serum 7αC4>52 ng/mL). Patients with ABAM, had faster colonic transit (CT), lower α diversity and a different microbial compositional profile based on β diversity compared with IBS-D without ABAM. There were no significant differences in the stool short-chain fatty acid (SCFA) concentrations between the two groups.1 There is evidence that transit impacts gut microbiome composition and diversity.2We wish to extend the previous analysis to compare the microbiome composition and SCFA in the same cohort of patients with IBS-D (total 181) with and without rapid CT, before and after adjusting for presence of ABAM. Patient selection, CT measurement, microbiome and SCFA analysis have been previously described.1CT was measured as geometric centre (GC) by scintigraphy (range 1–5, where 1=ascending colon, 5=stool). Patients with colonic GC at 24 hours >3.45 (90th percentile of normal3) were considered … ER -