RT Journal Article
SR Electronic
T1 IDDF2024-ABS-0186 Analysis of the correlation between gut microbiota and disease severity in patients with primary biliary cholangitis
JF Gut
JO Gut
FD BMJ Publishing Group Ltd and British Society of Gastroenterology
SP A252
OP A254
DO 10.1136/gutjnl-2024-IDDF.185
VO 73
IS Suppl 2
A1 Zhou, Jingping
A1 Li, Shuqi
A1 Chen, Meiya
A1 Zhou, Fei
A1 Xie, Chaohui
A1 Song, Yang
A1 Chen, Ligang
A1 Xu, Hongzhi
YR 2024
UL http://gut.bmj.com/content/73/Suppl_2/A252.abstract
AB Background To explore the correlation between gut microbiota and disease severity in patients with primary biliary cholangitis (PBC).Methods According to the natural course of the disease, 18 PBC patients were divided into two groups: early stage (asymptomatic stage and symptomatic stage) and decompensated stage. Ten patients with decompensated hepatitis B cirrhosis, 10 patients with decompensated alcoholic cirrhosis, and 10 healthy people were selected as controls. 16sRNA sequencing was performed in the fresh feces of the research subjects. To compare the composition of gut microbiota among decompensated PBC and early-stage PBC as well as other common decompensated chronic liver disease patients. Correlation analysis was conducted with clinical indicators.Results The diversity and abundance of gut microbiota in PBC patients decreased significantly compared to the healthy control group. The composition of the gut microbiota in the early PBC group was similar to that of the healthy control group, while the composition of the gut microbiota in the decompensated PBC group was less similar to that of the healthy control group (figure 1). Upon correlating clinical indicators with gut microbiota, it was revealed that genera such as Roseburia, Agathobacter, and Ruminococcus were found to be negatively correlated with the Child-Pugh score and total bilirubin (TBIL) levels, while positively correlated with albumin levels (figure 2). Furthermore, employing LEfSe for all groups comparisons, a total of 8 potential biomarkers at the genus level were identified: Agathobacter and Fusicatenibacter in the healthy control; Ligilactobacillus and Roseburia in the early PBC group; Veillonella and Klebsiella in the decompensated PBC group; Dialister in the decompensated hepatitis B cirrhosis group; Faecalibacterium in the decompensated alcoholic cirrhosis group (figure 3).Abstract IDDF2024-ABS-0186 Figure 1 (a)(b) The α-diversity analysis (ACE, Shannon) of the gut microbiota among PBC patients and healthy controls. (c)The β-diversity analysis of the gut microbiota among PBC patients and healthy controlsAbstract IDDF2024-ABS-0186 Figure 2 Correlations of altered gut microbiota with clinical characteristics in PBC patientsAbstract IDDF2024-ABS-0186 Figure 3 Key taxonomic differences of gut microbiota among the five groupsConclusions The gut microbiota of PBC patients exhibit dysbiosis, suggesting its involvement in the pathogenesis of the disease. Gut microbiota may potentially distinguish PBC from other common decompensated chronic liver diseases. Certain specific gut microbiota may be associated with disease progression.